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Polycationic supports and nucleic acid purification separation and hybridization

  • US 5,599,667 A
  • Filed: 09/23/1994
  • Issued: 02/04/1997
  • Est. Priority Date: 03/02/1987
  • Status: Expired due to Term
First Claim
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1. A method for purifying a polynucleotide greater than 100 nucleotides in length from an impure biological sample comprising an oligonucleotide 10 to 100 nucleotides in length comprising the steps of:

  • (a) contacting said biological sample with a solid support comprising a plurality of cations selected from the group consisting of ammonium, immonium and guanidinium ions, wherein said polynucleotide binds to said solid support and said oligonucleotide does not bind to said solid support, wherein said polynucleotide is at least three times greater in length than skid oligonucleotide; and

    (b) separating said oligonucleotide from said solid support while said polynucleotide is held to said solid support.

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