×

DNA sequencing by stepwise ligation and cleavage

  • US 5,599,675 A
  • Filed: 03/24/1995
  • Issued: 02/04/1997
  • Est. Priority Date: 04/04/1994
  • Status: Expired due to Term
First Claim
Patent Images

1. A method for determining a nucleotide sequence of a polynucleotide, the method comprising the steps of:

  • (a) ligating a probe to an end of a polynucleotide, the probe having a nuclease recognition site of a nuclease whose cleavage site is separate from its recognition site;

    (b) identifying one or more nucleotides at the end of the polynucleotide by the identity of the probe ligated thereto or by extending a strand of the polynucleotide or probe;

    (c) cleaving the polynucleotide with a nuclease recognizing the nuclease recognition site of the probe such that the polynucleotide is shortened by one or more nucleotides; and

    (d) repeating said steps (a) through (c) until said nucleotide sequence of the polynucleotide is determined.

View all claims
  • 4 Assignments
Timeline View
Assignment View
    ×
    ×