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Immunoassays for prostate specific antigen

  • US 5,599,677 A
  • Filed: 12/29/1993
  • Issued: 02/04/1997
  • Est. Priority Date: 12/29/1993
  • Status: Expired due to Term
First Claim
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1. An immunoassay method for detecting or quantitating an Analyte ("A") in a sample, the Analyte being capable of existing in the sample in a free form ("free Analyte") or bound to a Binding Molecule to form a complex of the Analyte and the Binding Molecule ("{(A)(Binding Molecule)}"), the method comprising the steps of:

  • (a) contacting the Analyte to an antibody ("α

    HE") which can bind the free Analyte but not {(A)(Binding Molecule)}, a capture antibody ("α

    nHE") which can bind both the free Analyte and {(A)(Binding Molecule)}, and a probe antibody ("α

    A*") specific for the Analyte, providing that if the probe antibody is a polyclonal antibody then the Analyte is contacted to the capture antibody before the probe antibody;

    (b) correlating the presence or amount of α

    A* which is bound to the Analyte, with the presence or amount of the Analyte in the sample, or correlating the amount of unbound α

    A* with the absence or amount of the Analyte in the sample.

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