Activation-state-specific phosphoprotein immunodetection

• US 5,599,681 A
• Filed: 10/13/1994
• Issued: 02/04/1997
• Est. Priority Date: 04/10/1992
• Status: Expired due to Term

• Current Assignee: Dana-Farber Cancer Institute
• Original Assignee: Dana-Farber Cancer Institute
• Inventors: Stiles, Charles D., Epstein, Richard J.
• Primary Examiner(s): Scheiner, Toni R.
• Application Number:
  US08/324,421
• Time in Patent Office: 845 Days
• Field of Search: 435/7.23, 435/7.4, 436/547, 436/543, 436/548, 530/387.7, 530/387.9, 530/388.8, 530/388.85, 530/389.7
• US Class Current: 435/7.23
• CPC Class Codes: C07K 16/28 against receptors, cell surface antigens or cell surface determinants
• CPC Class Codes: C07K 16/32 against translation products of oncogenes
• CPC Class Codes: C07K 16/40 against enzymes
• CPC Class Codes: C07K 2317/34 Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues

Abstract

Activation-state-specific and protein-specific antiphosphoprotein antibodies and methods for their production are disclosed. Also disclosed are methods for evaluating the prognosis and therapeutic outcome for patients using the antiphosphoprotein antibodies and methods for characterizing the activation state of a reversibly phosphorylated protein, kits including the antibodies for use in characterizing the activation state of a protein, and methods for evaluating the agonist or antagonist activity of pharmaceutically useful compounds towards the conversion of a specific protein from its inactive to its active state.

Claims

1. A method for producing antibody that specifically binds to one of the two isoforms of a reversibly phosphorylated protein and does not bind to the other isoform of said protein nor to proteins other than said protein, said method comprising the steps of:

a) providing a peptide consisting essentially of a reversible phosphorylation site of said protein, a phosphorylatable amino acid in said reversible phosphorylation site being in the phosphorylation state of one of the two isoforms of said protein, said phosphorylation state having been achieved chemically and not enzymatically;

b) raising antibodies against said peptide;

c) isolating a population of antibodies reactive with said peptide;

d) screening said population for antibodies not reactive with said peptide wherein said peptide is in the phosphorylation state of the other isoform of said protein, said phosphorylation state having been achieved chemically and not enzymatically;

e) collecting antibodies identified in screening step (d); and

f) testing antibodies collected in step (e) to verify that the antibodies specifically bind said protein and not proteins other than said protein.

2. The method of claim 1 wherein said two isoforms represent functionally divergent forms of said protein.

3. The method of claim 1 wherein said two isoforms represent active and inactive forms of said protein.

4. The method of claim 3 wherein said protein is active in the phosphorylated state.

5. The method of claim 3 wherein the protein is inactive in the phosphorylated state.

6. The method of claim 1 wherein the protein is phosphorylated on a tyrosine residue.

7. The method of claim 1 wherein the protein is phosphorylated on a serine and/or threonine residue.

8. The method of claim 1 wherein the protein is a tyrosine kinase.

9. The method of claim 1 wherein the protein is a serine or threonine kinase.

10. The method of claim 1 wherein the protein is a receptor.

11. The method of claim 10 wherein said receptor is a hormone receptor.

12. The method of claim 10 wherein said receptor is a growth factor receptor.

13. The method of claim 10 wherein said receptor is the c-erbB-2 receptor.

14. Antibody that specifically binds to a reversible tyrosine phosphorylation site of a reversibly phosphorylated protein in its phosphorylated isoform and does not bind to the non-phosphorylated isoform of said protein, nor to proteins other than said protein.

15. Antibody that specifically binds to a reversible tyrosine phosphorylation site of a reversibly phosphorylated protein in its non-phosphorylated isoform and does not bind to the phosphorylated isoform of said protein, nor to proteins other than said protein.

16. Antibody that specifically binds to a reversible phosphorylation site of the c-erbB-2 receptor in its active form and does not bind to the inactive form of said c-erbB-2 receptor, nor to proteins other than said c-erbB-2 receptor.

17. A method for screening for metastatic potential of tumors in patients with node-negative breast cancer comprising

reacting the antibody of claim 16 with tumor tissue from said patient;

detecting the extent of binding of said antibody to said tissue; and

correlating the extent of binding of said antibody with metastatic potential.

18. A kit for use in identifying the activation state of a reversibly phosphorylated protein in a sample by determining whether said protein is in its active or inactive isoform, said kit comprising

components required for extraction of said sample;

and

components necessary for use in a binding assay, said binding assay components comprising

antibody that specifically binds to a reversible phosphorylation site of the active isoform of said protein and does not bind to the inactive isoform of said protein nor to proteins other than said protein.

20. The kit of claim 18 or claim 19 wherein said antibody is a monoclonal antibody.

21. The kit of claim 18 or 19 wherein said components necessary for use in a binding assay further comprise a second indicator antibody linked to an indicator reagent.

22. The kit of claim 21 wherein said indicator reagent is selected from a group consisting of fluorescent, colorimetric, immunoperoxidase and isotopic reagents.

19. A kit for use in identifying the activation state of a reversibly phosphorylated protein in a sample by determining whether said protein is in its active or inactive isoform, said kit comprising

components required for extraction of said sample;

and

components necessary for use in a binding assay, said binding assay components comprising

antibody that specifically binds to a reversible phosphorylation site of the inactive isoform of said protein and does not bind to the active isoform of said protein nor to proteins other than said protein.