Advanced synchronous luminescence system

• US 5,599,717 A
• Filed: 09/02/1994
• Issued: 02/04/1997
• Est. Priority Date: 09/02/1994
• Status: Expired due to Term

• Current Assignee: Martin Marietta Energy Systems, Inc.
• Original Assignee: Martin Marietta Energy Systems, Inc.
• Inventors: Vo-Dinh, Tuan
• Primary Examiner(s): SNAY, JEFFREY R
• Application Number:
  US08/300,202
• Time in Patent Office: 886 Days
• Field of Search: 436/63, 436/64, 436/171, 436/172, 422/82.05, 422/82.08, 128/633, 128/634
• US Class Current: 436/63
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: G01J 3/1256 using acousto-optic tunable filter; acousto-optic elements or systems G02F1/11, G02F1/33
• CPC Class Codes: G01N 21/6402 Atomic fluorescence; Laser induced fluorescence
• CPC Class Codes: G01N 21/6456 Spatial resolved fluorescence measurements; Imaging
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence

Abstract

A method and apparatus for determining the condition of tissue or otherwise making chemical identifications includes exposing the sample to a light source, and using a synchronous luminescence system to produce a spectrum that can be analyzed for tissue condition.

Claims

1. A method of testing a tissue sample to determine malignant or pre-malignant abnormality, comprising the steps of:

exposing the tissue sample to an excitation radiation and thereby generating an emission radiation;

synchronously scanning the wavelength of the excitation radiation and the wavelength of the emission radiation to produce a spectrum; and

correlating the spectrum to a malignancy condition of the tissue sample.

2. The method according to claim 1, wherein the synchronously scanning step comprises maintaining a constant interval between the wavelength of the excitation radiation and the emission radiation during synchronous scanning.

3. The method according to claim 1, wherein the exposing step comprises directing an output beam from a laser light source to the tissue sample through at least one optical fiber.

4. The method according to claim 3, further comprising directing the output of the laser light source into a probe disposed in vivo next to the tissue sample.

5. The method according to claim 3, wherein the scanning step further comprises directing the output beam of the laser source through a dye module having a plurality of dyes, and changing the dyes to achieve a range of excitation wavelengths.

6. The method according to claim 3, wherein the scanning step further comprises directing the output beam of the laser source through a dye container, and selectively moving a plurality of dyes into the dye container to achieve a range of excitation wavelengths.

7. The method according to claim 1, wherein the synchronous scanning step comprises directing the excitation radiation into a first acousto-optic tunable filter, and changing an input radio frequency of the first filter to achieve a range of excitation wavelengths.

8. The method according to claim 7, wherein the synchronous scanning step further comprises directing the emission radiation into a second acousto-optic tunable filter, and changing an input radio frequency of the second filter to achieve a range of emission wavelengths.

9. The method according to claim 7, wherein the synchronous scanning step further comprises directing the emission radiation into the first filter at an angle relative to the excitation radiation.

10. The method according to claim 7, wherein the synchronous scanning step further comprises directing the emission radiation into the first filter, alternately providing two radio frequency signals to the first filter to alternately pass the excitation and emission radiations through the first filter, and alternately detecting the emission radiation when the first filter is not transmitting the excitation radiation.