Method of detecting abnormally phosphorylated tau(.tau.)

• US 5,601,985 A
• Filed: 06/27/1995
• Issued: 02/11/1997
• Est. Priority Date: 08/14/1991
• Status: Expired due to Term

• Current Assignee: Pennsylvania University of The Trustees of The
• Original Assignee: Trustees Of The University Of Pennsylvania (University of Pennsylvania)
• Inventors: Lee, Virginia M-Y., Trojanowski, John Q.
• Primary Examiner(s): Knode, Marian C.
• Assistant Examiner(s): DUFFY, PATRICIA ANN
• Application Number:
  US08/495,308
• Time in Patent Office: 595 Days
• Field of Search: 435/7.1, 435/7.9, 435/7.92, 435/7.93, 435/7.94, 435/7.95, 436/518
• US Class Current: 435/7.1
• CPC Class Codes: G01N 2474/20 Immunohistochemistry assay
• CPC Class Codes: G01N 2800/2821 Alzheimer
• CPC Class Codes: G01N 33/6896 Neurological disorders, e.g. Alzheimer's disease
• CPC Class Codes: Y10S 436/811 Test for named disease, body condition or organ function

Abstract

Antibodies specifically reactive with an abnormally phosphorylated peptide comprising SEQ ID NO: 3 wherein a serine in SEQ ID NO: 3 is abnormally phosphorylated are provided. Methods of detecting abnormally phosphorylated τ in a test sample and methods of diagnosing Alzheimer'"'"'s Disease with these antibodies are also provided.

Claims

1. A method of detecting abnormally phosphorylated τ

in a test sample, said method comprising;

(a) contacting a test sample with an antibody which specifically reacts with τ

which has an abnormally phosphorylated serine in the sequence LysSerProVal (SEQ ID NO;

3); and

,

(b) detecting the presence of said antibody bound to said τ

.

2. The method of claim 1 wherein the test sample comprises cerebrospinal fluid.

3. The method of claim 1 wherein said antibody is a monoclonal antibody.

4. The method of claim 1 wherein said antibody is detectably labeled.