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Methods for sorting polynucleotides using oligonucleotide tags

  • US 5,604,097 A
  • Filed: 12/19/1994
  • Issued: 02/18/1997
  • Est. Priority Date: 10/13/1994
  • Status: Expired due to Term
First Claim
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1. A method of sorting polynucleotides from a population of polynucleotides onto one or more solid phase supports, the method comprising the steps of:

  • (a) attaching an oligonucleotide tag from a repertoire of tags to each polynucleotide in a population of polynucleotides (i) such that substantially all different polynucleotides in the population have different oligonucleotide tags attached and (ii) such that each oligonucleotide tag from the repertoire comprises a plurality of subunits and each subunit of the plurality consists of an oligonucleotide having a length from three to six nucleotides or from three to six basepairs, the subunits being selected from a minimally cross-hybridizing set, wherein a subunit of the set and a complement of any other subunit of the set would have at least two mismatches and(b) sorting the polynucleotides from the population by specifically hybridizing the oligonucleotide tags with their respective complements, the respective complements being attached as uniform populations of substantially identical complements in spacially discrete regions on the one or more solid phase supports.

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