Luciferase, gene encoding the same and production process of the same

• US 5,604,123 A
• Filed: 06/15/1994
• Issued: 02/18/1997
• Est. Priority Date: 08/09/1988
• Status: Expired due to Term

• Current Assignee: Toray Industries Incorporated
• Original Assignee: Toray Industries Incorporated
• Inventors: Kazami, Jun, Nakamura, Haruji, Goto, deceased, Toshio
• Primary Examiner(s): Wax, Robert A.
• Assistant Examiner(s): Moore, William W.
• Application Number:
  US08/260,042
• Time in Patent Office: 979 Days
• Field of Search: 435/189, 435/69.1, 435/252.3, 435/252.33, 435/320.1, 536/23.2
• US Class Current: 435/189
• CPC Class Codes: C12N 9/0069 acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
• CPC Class Codes: C12Q 1/6897 involving reporter genes operably linked to promoters
• CPC Class Codes: C12Y 113/12006 Cypridina-luciferin 2-monooxygenase (1.13.12.6), i.e. cypridina-luciferase

Abstract

The present invention provides luciferase with an amino acid sequence shown in FIG. 1, a gene encoding the the same, a recombinant vector DNA comprising the gene ligated at a site downstream of a promoter which can be expressed in a host cell, a transformant prepared by transforming the host cell with the recombinant vector DNA and a process of producing luciferase using the transformant.

Claims

1. An isolated DNA fragment comprising a sequence encoding a luciferase of Cypridina hilgendorfii having an amino acid sequence set forth in FIG. 1 selected from the group consisting of positions 1 through 555, positions 29 through 555, positions 30 through 555, positions 31 through 555, and positions 32 through 555.

2. The DNA sequence of claim 1 having the nucleotide sequence shown in FIG. 1 and which encodes a luciferase of Cypridina hilgendorfii.

3. A vector DNA comprising the DNA sequence of claim 1 encoding a luciferase of Cypridina hilgendorfii ligated at a site operably linked downstream of a promoter which can be expressed in a host cell.

4. A vector DNA comprising the DNA sequence of claim 1 encoding a luciferase of Cypridina hilgendorfii ligated at the site operably linked downstream of a promoter and a ribosome binding site, which can be expressed in E. coli.

5. A transformant prepared by transforming a host cell selected from the group consisting of an animal cell, a yeast cell and an E. coli cell with a vector according to claim 3.

6. A process for producing luciferase comprising culturing the transformant according to claim 5 and thereafter recovering said luciferase from the culture.