Thermophilic fungal expression system

• US 5,604,129 A
• Filed: 06/01/1995
• Issued: 02/18/1997
• Est. Priority Date: 07/20/1994
• Status: Expired due to Term

• Current Assignee: Novozymes
• Original Assignee: NOVO Nordisk Biotech Incorporated (Novo Nordisk A/S)
• Inventors: Boominathan, Karuppan C., Jensen, Ejner B.
• Primary Examiner(s): Ketter, James S.
• Application Number:
  US08/457,201
• Time in Patent Office: 628 Days
• Field of Search: 435/254.1, 435/254.11, 435/254.3, 435/122.3, 435/320.1, 435/69.1, 435/911
• US Class Current: 435/254.11
• CPC Class Codes: C12N 15/80 for fungi
• CPC Class Codes: C12N 9/248 Xylanases
• CPC Class Codes: Y10S 435/911 using fungi

Abstract

The present invention relates to recombinant thermophilic host cells comprising a nucleic acid sequence encoding a heterologous protein, and a method of producing recombinant protein utilizing same. The recombinant hosts of the present invention provide a better morphology in tank fermentations than many known fungal host cells, such as Aspergillus, which morphology results in lower viscosity levels, and therefore improved productivity.

Claims

1. A recombinant Myceliophthora host strain which produces, in tank fermentation, about 80% or less of the viscosity produced by Aspergillus oryzae cultured under identical conditions.

2. The strain of claim 1 which produces, in tank fermentation, about 50% or less of the viscosity produced by Aspergillus oryzae cultured under identical conditions.

3. The strain of claim 1 which produces, in tank fermentation, about 30% or less of the viscosity produced by Aspergillus oryzae cultured under identical conditions.

4. The cell of claim 1 which is obtained from a thermophilic fungus.

5. The strain of claim 1 which is obtained from Myceliophthora thermophilum.