Releasable multiwell plate cover

• US 5,604,130 A
• Filed: 05/31/1995
• Issued: 02/18/1997
• Est. Priority Date: 05/31/1995
• Status: Expired due to Term

• Current Assignee: Molecular Devices, Siemens Healthcare Diagnostics Incorporated (Siemens AG)
• Original Assignee: Chiron Corporation (Bayer AG), LJL Biosystems, Inc. (Molecular Devices)
• Inventors: Nordell, Benjamin T., Richardson, Bruce J., Warner, Brian D., El-Hage, Amer
• Primary Examiner(s): BEISNER, WILLIAM H
• Application Number:
  US08/451,025
• Time in Patent Office: 629 Days
• Field of Search: 435/283.1, 435/286.1, 435/286.2, 435/286.7, 435/287.2, 435/287.3, 435/288.3, 435/288.4, 435/288.7, 435/303.1, 435/303.3, 435/305.2 , 435/305.3, 435/808, 422/63, 422/65, 422/99, 422/101, 422/102, 220/23.2, 220/23.4, 220/23.8, 220/23.83, 220/523, 220/526, 220/255, 220/359, 220/378, 100/54, 100/92, 100/211, 428/40, 428/163, 428/167, 428/172, 428/411.1, 428/446, 428/447, 428/450, 428/451, 428/465, 359/396, 359/398, 156/69, 277/207 R, 277/211, 277/213
• US Class Current: 435/286.7
• CPC Class Codes: B01L 3/50825 Closing or opening means, corks, bungs closures for containers B65D; means for removing stoppers B67B7/02
• CPC Class Codes: B01L 3/50851 specially adapted for heating or cooling samples laboratory heating apparatus B01L7/00; incubators C12M
• CPC Class Codes: B01L 3/50853 with covers or lids

Abstract

A cover effective to releasably seal a multiwell container, such as a microtitration plate, is disclosed. The cover contains a pad, fashioned from a flexible polymer sheet, and a plurality of resiliently compressible ridges formed on the sheet. The ridges are deformable, such that application of pressure applied to the cover is effective to form a fluid-tight seal between the pad and the well openings. The ridges extend from the pad sufficiently to break the seal upon release of the pressure.

Claims

1. A pad for use in sealing wells having openings in the upper surface of a multi-well plate, comprising

a flexible polymer sheet defining a planar expanse adapted to cover the wells in the plate, and

formed on said expanse, a plurality of resiliently compressible ridges adapted to seat over the openings of said wells, with the pad placed operatively over the plate,

said ridges being deformable, with application of a substantially uniform pressure applied to the side of the sheet opposite said expanse, to form a substantially fluid-tight seal between said expanse and such well openings, and

said ridges extending from said expanse sufficiently to break said seal upon release of said pressure.

2. The pad of claim 1, wherein said sheet and ridges are formed integrally of a compressible rubber material, said sheet has a thickness between about 90 and 150 mils, and said ridges in a relaxed state extend between about 0.005 and 0.030 inches from the surface of said planar expanse.

3. The pad of claim 2, wherein said rubber material is silicon rubber or polyurethane rubber.

4. The pad of claim 2, which further includes a hydrophobic film covering said expanse and ridges, and having a thickness between about 1-4 mils.

5. The pad of claim 4, wherein said film contains high density polyethylene (HDPE).

6. The pad of claim 2, wherein said pad further includes a hydrophobic film disposed between said expanse and said well openings, and having a thickness between about 2-5 mils.

7. The pad of claim 6, wherein said film contains high density polyethylene (HDPE).

8. The pad of claim 1, for use with a plate whose well openings are substantially coplanar with the surface of the plate, wherein said ridges are discontinuous across surface regions of said expanse corresponding to surface regions of the plate between well openings.

9. The pad of claim 1, for use with a plate whose well openings are defined by raised rims extending from the surface of the plate.

10. The pad of claim 9, wherein the ridges form a rectangular array on the expanse, and points of ridge intersections in the array correspond to positions of well openings in the plate.

11. In an automated plate handling apparatus of the type having a tray for receiving a multiwell plate having a plurality of wells with upper planar openings, sample-handling means for heating or shaking the wells, and a sealing assembly including a cover and means for moving said cover from a retracted position toward a sealing position, a pad attached to said cover for use in sealing the wells of said plate, with the cover moved toward its sealing position, at which the pad is pressed against the surface of the plate, where in the improvement comprises, said pad comprising

a flexible polymer sheet defining a planar expanse dimensioned to cover the wells in the plate, and

formed on said expanse, a plurality of resiliently compressible ridges adapted to seat over the openings of said wells,

said ridges being deformable, as the cover is moved toward its sealing position, to form a substantially fluid-tight seal between said expanse and such well openings,

said ridges extending from said expanse sufficiently to break said seal upon movement of the cover toward its retracted position.

12. The apparatus of claim 11, wherein said sheet and ridges are formed integrally of a compressible rubber material, said sheet has a thickness between about 90 and 150 mils, and said ridges in a relaxed state extend between about 5 and 30 mils from the surface of said planar expanse.

13. The apparatus of claim 12, wherein said rubber material is silicon rubber or polyurethane rubber.

14. The apparatus of claim 12, which further includes a hydrophobic film covering said expanse and ridges, and having a thickness between about 1-4 mils.

15. The apparatus of claim 14, wherein said film contains high density polyethylene (HDPE).

16. The apparatus of claim 12, wherein said pad further includes a hydrophobic film disposed between said expanse and said well openings, and having a thickness between about 2-5 mils.

17. The apparatus of claim 16, wherein said film contains high density polyethylene (HDPE).

18. The apparatus of claim 11, for use with a plate whose well openings are defined by raised rims extending from the surface of the plate.

19. The apparatus of claim 18, wherein the ridges form a rectangular array on the expanse, and points of ridge intersections in the array correspond to positions of well openings in the plate.

20. The apparatus of claim 11, for use with a plate whose well openings are substantially coplanar with the surface of the plate, wherein said ridges are discontinuous across surface regions of said expanse corresponding to surface regions of the plate between well openings.

21. The apparatus of claim 11, wherein the plate handling apparatus is a luminometer.