Recombinant human basic fibroblast growth factor

• US 5,604,293 A
• Filed: 04/01/1994
• Issued: 02/18/1997
• Est. Priority Date: 09/12/1985
• Status: Expired due to Term

• Current Assignee: Scios, Inc. (Johnson & Johnson)
• Original Assignee: Scios, Inc. (Johnson & Johnson)
• Inventors: Abraham, Judith A., Fiddes, John C.
• Primary Examiner(s): Ulm, John
• Assistant Examiner(s): SAOUD, CHRISTINE J
• Application Number:
  US08/221,462
• Time in Patent Office: 1,054 Days
• Field of Search: 530/399, 530/350, 514/12, 930/10
• US Class Current: 530/399
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/501 acidic FGF [aFGF]
• CPC Class Codes: C07K 14/503 basic FGF [bFGF]
• CPC Class Codes: C12N 15/67 General methods for enhancing the expression

Abstract

The DNA sequences encoding human and bovine acidic and basic fibroblast growth factors (FGF) can be recombinantly expressed to obtain practical amounts of proteins useful in effecting wound healing and related tissue repair.

Claims

1. Biologically active recombinant human basic fibroblast growth factor in homogeneous, purified and isolated form, free from any infectious impurities, and free from substances normally accompanying native human basic fibroblast growth factor and from post-translational modifications of the cysteines of native human basic fibroblast growth factor.

2. The recombinant human basic fibroblast growth factor of claim 1 wherein said growth factor has the amino acid sequence numbered 1-146 in FIG. 4.

3. The recombinant human basic fibroblast growth factor of claim 1 wherein said growth factor has the amino acid sequence numbered 16-146 in FIG. 4.

4. The recombinant human basic fibroblast growth factor of claim 1 wherein said growth factor has the amino acid sequence numbered (-9) or (-8) to 146 in FIG. 4.