Reversible charge-based sequestration on solid support
First Claim
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1. A method for modifying a substrate compound, the method comprising:
- (i) providing, in a reaction mixture, a substrate compound comprising a reactive moiety;
(ii) contacting the reactive moiety with a reactant to form a modified substrate compound;
(iii) providing a solid support, wherein one of said substrate compound and said solid support comprises a selectively chargeable moiety (SCM) having a non-associating charge state;
(iv) altering the non-associating charge state of the selectively chargeable moiety to an associating charge state;
(v) contacting the modified substrate compound with the solid support such that the modified substrate compound is sequestered by the solid support; and
(vi) altering the associating charge state of the selectively chargeable moiety to a non-associating charge state such that the modified substrate compound is not sequestered by the solid support.
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Abstract
Methods for reversibly associating a substrate compound and a solid support are described. In general, the methods feature the use of selectively chargeable moieties. Apparatus for use with the methods of the invention is also described.
23 Citations
23 Claims
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1. A method for modifying a substrate compound, the method comprising:
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(i) providing, in a reaction mixture, a substrate compound comprising a reactive moiety; (ii) contacting the reactive moiety with a reactant to form a modified substrate compound; (iii) providing a solid support, wherein one of said substrate compound and said solid support comprises a selectively chargeable moiety (SCM) having a non-associating charge state; (iv) altering the non-associating charge state of the selectively chargeable moiety to an associating charge state; (v) contacting the modified substrate compound with the solid support such that the modified substrate compound is sequestered by the solid support; and (vi) altering the associating charge state of the selectively chargeable moiety to a non-associating charge state such that the modified substrate compound is not sequestered by the solid support. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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19. A method for reversibly associating a compound with a solid support, the method comprising:
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(i) providing, in a reaction mixture, a compound including a selectively chargeable moiety (SCM) which can be reversibly oxidized between an associating charge state which can associate with the solid support and a non-associating charge state which associates less with the solid support; (ii) contacting the reaction mixture containing the compound with a solid support under conditions that the SCM is in the associating charge state and the compound associates with the solid support; (iii) separating the solid support from at least a portion of the mixture; and (iv) altering the charge state of the SCM to a non-associating charge state such that the compound dissociates from the solid support.
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20. A method for modifying a substrate compound, the method comprising:
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(i) providing, in a reaction mixture, a substrate compound comprising a selectively chargeable moiety having a non-associating charge state, and a reactive moiety; (ii) contacting the reactive moiety with a reactant to form a modified substrate compound; (iii) altering, by an electrochemical redox reaction, the non-associating charge state the selectively chargeable moiety to an associating charge state; (iv) contacting the selectively chargeable moiety with a solid support such that the modified substrate compound is sequestered by the solid support; (v) separating the solid support from the at least a portion of the mixture; and (vi) altering, by an electrochemical redox reaction, the associating charge state of the selectively chargeable moiety to a non-associating charge state such that the modified substrate compound is not sequestered by the solid support.
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21. A method for reversibly sequestering a substrate compound on a solid support, the method comprising:
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(i) providing in a reaction mixture, a substrate compound comprising a selectively chargeable moiety; (ii) reacting the substrate compound with a reactant; (iii) contacting the reaction mixture with a means for altering an oxidation state of the chargeable moiety from a state which does not bind to the solid support to a state which does bind to the solid support; (iv) contacting the substrate compound with the solid support such that the substrate compound becomes sequestered by the support; (v) disposing the support in a second reaction mixture; (vi) contacting the reaction mixture with a means for altering an oxidation state of the chargeable moiety from a state which does bind to the solid support to a state which does not bind to the solid support;
such that the substrate compound is not sequestered by the support. - View Dependent Claims (22)
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23. A method for synthesizing a synthetic product, the method comprising:
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(i) providing in a reaction mixture, a substrate compound comprising a reactive moiety and a selectively chargeable moiety (SCM) having a non-associating charge state; (ii) contacting the reactive moiety with a reactant to form a modified substrate compound including a synthetic product; (iii) altering the non-associating charge state of the selectively chargeable moiety to an associating charge state; (iv) contacting the modified substrate compound with a solid support such that the modified substrate compound is sequestered by the solid support; (v) altering the associating charge state of the selectively chargeable moiety to a non-associating charge state such that the modified substrate compound is not sequestered by the solid support; and (vi) cleaving the synthetic product from the substrate compound.
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Specification