Active programmable electronic devices for molecular biological analysis and diagnostics
First Claim
1. An electronic device adapted to receive a solution comprising:
- a substrate,a plurality of selectively addressable electrodes, the electrodes being disposed upon the substrate and electrically insulated from one another prior to receipt of the solution,a current source for providing a selective current for the electrodes, individual buffer reservoirs associated with said electrodes, andindividual permeation layers disposed adjacent said individual buffer reservoirs, forming addressable binding locations.
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0 Petitions
Accused Products
Abstract
A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridization, antibody/antigen reaction, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micromachining techniques. The device can electronically control the transport and attachment of specific binding entities to specific micro-locations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific microlocations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.
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Citations
47 Claims
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1. An electronic device adapted to receive a solution comprising:
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a substrate, a plurality of selectively addressable electrodes, the electrodes being disposed upon the substrate and electrically insulated from one another prior to receipt of the solution, a current source for providing a selective current for the electrodes, individual buffer reservoirs associated with said electrodes, and individual permeation layers disposed adjacent said individual buffer reservoirs, forming addressable binding locations. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. An electronic device adapted to receive a solution including electrolytes and one or more charged specific binding entities, the device being adapted to affect the electrophoretic transport of the specific binding entities in the solution to selected locations on the device surface, the device comprising:
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a substrate, a first electrode, the electrode being supported by the substrate, the electrode being adapted to receive current, a permeation layer having selective diffusion properties for the passage from the solution to the electrode of the electrolytes relative to the charged specific binding entities, permitting the free transport of counter-ions through the permeation layer to the electrode, the permeation layer being disposed adjacent the first electrode, the permeation layer and the first electrode being separated by a buffer reservoir, an attachment layer adjacent to the permeation layer with selective binding properties for the specific binding entities, and a second electrode, the second electrode being electrically insulated from the first electrode prior to the application of the solution. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23)
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24. An electronic device adapted to receive a solution including electrolytes and one or more charged specific binding entities, the device being adapted to affect the electrophoretic transport of the specific binding entities in the solution to selected locations on the device surface, the device comprising:
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a substrate, a first electrode, the electrode being supported by the substrate, the electrode being adapted to receive current, a permeation layer aminopropyltriethoxy silane, the permeation layer being disposed adjacent the first electrode, an attachment layer adjacent to the permeation layer with selective binding properties for the specific binding entities, and a second electrode, the second electrode being electrically insulated from the first electrode prior to the application of the solution. - View Dependent Claims (25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35)
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36. An electronic device adapted to receive a solution including electrolytes and one or more charged specific binding entities, the device being adapted to affect the electrophoretic transport of the specific binding entities in the solution to selected locations on the device surface, the device comprising:
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a substrate, a first electrode, the electrode being supported by the substrate, the electrode being adapted to receive current, a permeation layer having selective diffusion properties for the passage from the solution to the electrode of the electrolytes relative to the charged specific binding entities, permitting the free transport of counter-ions through the permeation layer to the electrode, the permeation layer being disposed adjacent the first electrode, an attachment layer adjacent to the permeation layer with selective binding properties for the specific binding entities, and an unaddressed microlocation, the first electrode being electrically insulated from the unaddressed microlocation prior to the application of the solution. - View Dependent Claims (37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47)
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Specification