DNA diagnostic based on mass spectrometry
First Claim
Patent Images
1. A process for detecting a target nucleic acid sequence present in a biological sample, comprising the steps of:
- a) obtaining a nucleic acid molecule containing a target nucleic acid sequence from a biological sample;
b) hybridizing a detector oligonucleotide with the target nucleic acid sequence, wherein at least one of the detector oligonucleotide or the target nucleic acid sequence has been conditioned;
c) removing unhybridized detector oligonucleotide;
d) ionizing and volatizing the product of step c); and
e) detecting the detector oligonucleotide by mass spectrometry, wherein detection of the detector oligonucleotide indicates the presence of the target nucleic acid sequence in the biological sample.
3 Assignments
0 Petitions
Accused Products
Abstract
The invention provides fast and highly accurate mass spectrometer based processes for detecting a particular nucleic acid sequence in a biological sample. Depending on the sequence to be detected, the processes can be used, for example, to diagnose (e.g. prenatally or postnatally) a genetic disease or chromosomal abnormality; a predisposition to a disease or condition (e.g. obesity, artherosclerosis, cancer), or infection by a pathogenic organism (e.g. virus, bacteria, parasite or fungus).
-
Citations
45 Claims
-
1. A process for detecting a target nucleic acid sequence present in a biological sample, comprising the steps of:
-
a) obtaining a nucleic acid molecule containing a target nucleic acid sequence from a biological sample; b) hybridizing a detector oligonucleotide with the target nucleic acid sequence, wherein at least one of the detector oligonucleotide or the target nucleic acid sequence has been conditioned; c) removing unhybridized detector oligonucleotide; d) ionizing and volatizing the product of step c); and e) detecting the detector oligonucleotide by mass spectrometry, wherein detection of the detector oligonucleotide indicates the presence of the target nucleic acid sequence in the biological sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
-
-
19. A process for detecting a target nucleic acid sequence present in a biological sample, comprising the steps of:
-
a) obtaining a nucleic acid molecule containing a target nucleic acid sequence from a biological sample; b) amplifying the target nucleic acid sequence, thereby obtaining an amplified target nucleic acid sequence; c) hybridizing a detector oligonucleotide with the amplified target nucleic acid sequence, wherein at least one of the detector oligonucleotide or the amplified target nucleic acid sequence has been conditioned; d) removing unhybridized detector oligonucleotide; e) ionizing and volatizing the product of step c); and f) detecting the detector oligonucleotide by mass spectrometry, wherein detection of the detector oligonucleotide indicates the presence of the target nucleic acid sequence in the biological sample. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
-
-
33. A process for detecting a target nucleic acid sequence present in a biological sample, comprising the steps of:
-
a) obtaining a target nucleic acid sequence from a biological sample; b) amplifying and conditioning the target nucleic acid sequence, thereby producing amplified and conditioned nucleic acid molecules; c) digesting the amplified and conditioned nucleic acid molecules using at least one appropriate nucleases, thereby producing conditioned fragments; d) ionizing and volatizing the conditioned fragments; and e) detecting the conditioned fragments to determine the presence of the target nucleic acid sequence. - View Dependent Claims (34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45)
-
Specification