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DNA diagnostic based on mass spectrometry

  • US 5,605,798 A
  • Filed: 03/17/1995
  • Issued: 02/25/1997
  • Est. Priority Date: 01/07/1993
  • Status: Expired due to Term
First Claim
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1. A process for detecting a target nucleic acid sequence present in a biological sample, comprising the steps of:

  • a) obtaining a nucleic acid molecule containing a target nucleic acid sequence from a biological sample;

    b) hybridizing a detector oligonucleotide with the target nucleic acid sequence, wherein at least one of the detector oligonucleotide or the target nucleic acid sequence has been conditioned;

    c) removing unhybridized detector oligonucleotide;

    d) ionizing and volatizing the product of step c); and

    e) detecting the detector oligonucleotide by mass spectrometry, wherein detection of the detector oligonucleotide indicates the presence of the target nucleic acid sequence in the biological sample.

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