Purified extract of uvaria brevistipitata and a process for obtaining the purified extract therefor

• US 5,607,673 A
• Filed: 04/20/1995
• Issued: 03/04/1997
• Est. Priority Date: 04/20/1995
• Status: Expired due to Fees

• Current Assignee: Cssaha Incorporated
• Original Assignee: Cssaha Incorporated
• Inventors: Bashengezi, Constantin M.
• Primary Examiner(s): Rollins, John W.
• Application Number:
  US08/425,747
• Time in Patent Office: 684 Days
• Field of Search: 424/195.1, 514/885, 514/894, 514/934
• US Class Current: 424/773
• CPC Class Codes: A61K 36/185 Magnoliopsida (dicotyledons)
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

In HIV infected individuals, certain clinical and biological markers are used to assess the progression or regression of the disease. From the plant, Uvaria bevistipitata of the Annonaceae family, a substantially pure extract was derived. This extract was administered to 268 HIV infected patients in a clinical trail in Zaire Africa and dramatic results were obtained. The extract was also submitted to a laboratory for in vitro analysis. when tested against the HIV reverse transcriptase enzyme, the extract tested 96.7% active. In further laboratory analysis, against HIV-_IIB, it demonstrated efficacy at doses that showed no cytotoxic effects.

Claims

1. The substantially purified extract of a plant root from the family Annonaceae and the genus Uvaria wherein the species is brevistipitata.

2. A method for the treatment of AIDS comprising administering to a patient in need thereof a substantially pure root extract taken from the plant family Annonaceae and the genus Uvaria in an amount effective to promote remission of said AIDS, in a pharmaceutically acceptable carrier therefor.

3. The method of claim 2 wherein the species is brevistipitata.

4. A method for treating immunodeficiency diseases including HIV, Hepatitis A and B, Herpes Simplex Viruses, and Lupus, comprising administering to a patient in need thereof an effective amount of a substantially pure root extract of a plant from the family Annonaceae and the species Uvaria brevistipitata comprising the steps of:

providing a substantially purified Annonaceae root extract from the species Uvaria brevistipitata.

5. The method of claim 4 wherein providing a substantially pure Annonaceae root extract from a species of Uvaria further comprises the steps of:

grinding a quantity of root from a Uvaria brevistipitata plant;

boiling said quantity of root in distilled water to make a solution;

cooling said solution to form a total extract solution;

adding a quantity of activated carbon to said total extract solution;

filtering said total extract solution to form a total extract filtrate;

distilling said total extract filtrate;

mixing a quantity of methanol with said filtrate to form a methanol/precipitate solution;

filtering said methanol/precipitate solution to recover a purified root extract;

collecting said purified root extract; and

,

drying said root extract.

6. The method of claim 4 wherein administering said root extract to said treatment subject further comprises the steps of:

administering a therapeutic dosage based on a range of dosages from 0.608 milligrams per kilogram per day to 0.68526 grams per kilogram per day;

providing a quantity of said root extract in accordance with said therapeutic dosage; and

ingesting said quantity of said root extract.

7. A process for obtaining substantially pure root extract plant from the family Annonaceae and the genus Uvaria comprising the steps of:

providing a quantity of root from an Annonaceae plant, genus Uvaria;

grinding a quantity of root from a Uvaria brevistipitata Plant;

boiling said quantity of root in distilled water to make a solution;

cooling said solution to form a total extract solution;

adding a quantity of activated carbon to said total extract solution;

filtering said total extract solution to form a total extract filtrate;

distilling said total extract filtrate;

mixing a quantity of methanol with said total extract filtrate to form a methanol/filtrate solution;

filtering said methanol/filtrate solution to collect a purified root extract;

collecting said purified root extract; and

,

drying said root extract.

8. The process of claim 7 wherein grinding a quantity of root from a Uvaria brevistipitata plant further comprises the steps of:

providing a conventional mill;

reducing said root to a pulpy consistency; and

,

disrupting plant cell walls of said root.

9. The process of claim 7 wherein boiling said quantity of root in distilled water to make a solution further comprises the step of:

boiling said quantity of root for thirty minutes.

10. The process of claim 7 wherein adding a quantity of activated carbon to said total extract solution further comprises the step of:

providing 100 grams of activated carbon.

11. The process of claim 7 wherein filtering said total extract solution to form a total extract filtrate further comprises the step of:

providing a filter element, said filter element comprising one member taken from the following group;

cotton gauze, filter paper, or cotton balls.

12. The process of claim 7 wherein the steps of distilling said total extract, filtering said total extract to form a total extract filtrate, mixing a quantity of methanol with said total extract filtrate to form a methanol/filtrate solution, filtering said methanol/filtrate solution to collect a purified root extract on the paper filter, and collecting said purified root extract are repeated.

13. The process of claim 12 wherein the step of adding a drying agent to said root extract further comprises the step of:

providing a quantity of Aerosol 200 brand drying agent.

14. The process of claim 7 wherein the step of drying said root extract further comprises the steps of:

adding a drying agent to said root extract;

grinding said root extract in the presence of said drying agent in a mortar and pestle;

and placing said ground root extract in an oven.