DNA photocleavage using texaphyrins
First Claim
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1. A method of light-induced cleavage of a polymer of deoxyribonucleic acid, the method comprising:
- contacting the polymer with a photosensitive texaphyrin; and
exposing the photosensitive texaphyrin to light for a time sufficient to cleave the polymer.
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Abstract
Methods of cleavage of a polymer of deoxyribonucleic acid using photosensitive texaphyrins are disclosed. A preferred method of use is the site-specific cleavage of a polymer of deoxyribonucleic acid and a preferred texaphyrin is a derivatized texaphyrin having binding specificity, in particular, a texaphyrin covalently coupled to a site-directing molecule, preferably an oligonucleotide.
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Citations
20 Claims
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1. A method of light-induced cleavage of a polymer of deoxyribonucleic acid, the method comprising:
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contacting the polymer with a photosensitive texaphyrin; and exposing the photosensitive texaphyrin to light for a time sufficient to cleave the polymer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. A method for targeted intracellular light-induced cleavage of DNA, the method comprising:
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introducing into a cell a photosensitive texaphyrin coupled to an oligonucleotide having complementary binding affinity for a targeted DNA, and exposing the photosensitive texaphyrin to light for a time sufficient to effect cleavage of the DNA. - View Dependent Claims (18, 19)
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20. A method for modulating the activity of a DNA, the method comprising:
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contacting a DNA with a photosensitive texaphyrin coupled to an oligonucleotide having complementary binding affinity for said DNA, and exposing the photosensitive texaphyrin to light for a time sufficient to effect cleavage of the DNA.
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Specification