Method and kit for destroying ability of nucleic acid to be amplified

• US 5,612,200 A
• Filed: 03/28/1994
• Issued: 03/18/1997
• Est. Priority Date: 06/24/1992
• Status: Expired due to Term

• Current Assignee: Gen-Probe Incorporated (Hologic Incorporated)
• Original Assignee: Gen-Probe Incorporated (Hologic Incorporated)
• Inventors: Kamisango, Keiichi, Kacian, Daniel L., Dattagupta, Nanibhushan, Ryder, Thomas B.
• Primary Examiner(s): Zitomer, Stephanie W.
• Application Number:
  US08/218,990
• Time in Patent Office: 1,086 Days
• Field of Search: 435/91.2, 435/91.52, 935/77, 935/78
• US Class Current: 435/91.2
• CPC Class Codes: C12Q 1/6848 characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
• CPC Class Codes: C12Q 2527/125 Specific component of sample, medium or buffer

Abstract

Method for destroying the ability of a nucleic acid to be amplified, comprising the step of contacting the nucleic acid with bleach in an amount and for a time sufficient to inhibit the ability of that nucleic acid to be amplified in an amplification reaction.

Claims

1. A method for inhibiting the ability to be amplified of nucleic acids produced by a previous amplification, comprising the step of:

contacting said nucleic acids produced by a previous amplification with bleach in an amount and for a time sufficient to inhibit the subsequent amplification of said nucleic acids produced by a previous amplification, thereby inhibiting the ability of said nucleic acids produced by a previous amplification to be amplified.

2. The method of claim 1 wherein said bleach is at a concentration between 1% and 50% for a time between 10 seconds and 15 hours at a temperature between 0°

C. and 95°

C.

6. The method of any one of claims 1, 2, 4, and 5, wherein said amplification is the polymerase chain reaction.

7. The method of any one of claims 1, 2, 4, and 5, wherein said amplification is polymerase driven amplification.

3. A kit for inhibiting the ability to be amplified of nucleic acids produced by a previous amplification comprising materials for nucleic acid amplification and bleach.

4. A method for reducing the nucleic acid amplification inhibitory effect of nucleic acids produced by a previous amplification comprising the step of:

contacting said nucleic acids produced by previous amplification method with bleach in an amount and for a time sufficient to reduce the nucleic acid amplification inhibitory effect of said nucleic acids,

thereby reducing the nucleic acid amplification inhibitory effect of said nucleic acids produced by a previous amplification on subsequent amplification of nucleic acids.

5. The method of claim 4 wherein said bleach is at a concentration between 1% and 50% for a time between 10 seconds and 15 hours at a temperature between 0°

C. and 95°

C.