Stimulation, production and culturing of hematopoietic progenitor cells by fibroblast growth factors

• US 5,612,211 A
• Filed: 06/15/1993
• Issued: 03/18/1997
• Est. Priority Date: 06/08/1990
• Status: Expired due to Term

• Current Assignee: Sloan-Kettering Institute For Cancer Research (Memorial Sloan-Kettering Cancer Center), New York University, Randolph Rand Corporation Of New York
• Original Assignee: Sloan-Kettering Institute For Cancer Research (Memorial Sloan-Kettering Cancer Center), New York University
• Inventors: Wilson, Elaine L., Gabrilove, Janice
• Primary Examiner(s): Chan, Christina Y.
• Assistant Examiner(s): Lukton, David
• Application Number:
  US08/076,875
• Time in Patent Office: 1,372 Days
• Field of Search: 530/399, 530/324, 530/351, 424/577, 435/240.2, 435/240.21, 435/240.25, 514/2, 514/12
• US Class Current: 435/378
• CPC Class Codes: A61K 2035/124 the cells being hematopoietic, bone marrow derived or blood cells
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 38/1825 Fibroblast growth factor [FGF]
• CPC Class Codes: C07K 14/503 basic FGF [bFGF]
• CPC Class Codes: C12N 2501/11 Epidermal growth factor [EGF]
• CPC Class Codes: C12N 2501/115 Basic fibroblast growth factor (bFGF, FGF-2)
• CPC Class Codes: C12N 2501/125 Stem cell factor [SCF], c-kit ligand [KL]
• CPC Class Codes: C12N 2501/15 Transforming growth factor beta (TGF-β)
• CPC Class Codes: C12N 2501/22 Colony stimulating factors (G-CSF, GM-CSF)
• CPC Class Codes: C12N 2501/39 Steroid hormones
• CPC Class Codes: C12N 2501/91 Heparin
• CPC Class Codes: C12N 2502/1394 Bone marrow stromal cells; whole marrow
• CPC Class Codes: C12N 5/0623 Stem cells
• CPC Class Codes: C12N 5/0663 Bone marrow mesenchymal stem cells (BM-MSC)

Abstract

Fibroblast growth factors are used in vivo, in situ and in vitro to stimulate stem cells, hemopoiesis, the immune system, transplant donor cells, culture and/or engraftment, wherein the use of fibroblast growth factors is disclosed for the stimulation of stem cells or hemopoietic cells, supporting cells and their progeny, in vitro, in situ and in vivo, as well as corresponding engrafting sites in vivo.

Claims

1. A method for in vitro proliferation of hematopoietic progenitor cells that are progenitors of mature blood or lymph cells comprising culturing the hematopoietic progenitor cells in a culture medium containing a hematopoietic progenitor cell proliferating effective amount of a fibroblast growth factor for a time sufficient to permit proliferation of the hematopoietic progenitor cells.

2. A method in accordance with claim 1, wherein said hematopoietic progenitor cells are obtained drom bone marrow.

3. A method according to claim 1, further comprising recovering the hematopoietic progenitor cells proliferated in said culturing step.

4. A method according to claim 1, wherein said fibroblast growth factor is selected from the group consisting of basic fibroblast growth factor, acidic fibroblast growth factor, an hst/K-fgf gene product, FGF-6, KGF, FGF-5 and int-2.

5. A method according to claim 1, wherein said fibroblast growth factor is a basic fibroblast growth factor.

6. A method according to claim 5 wherein said basic fibroblast growth factor is selected from the group consisting of a 17.5 kilodalton, a 22.5 kilodalton, a 23.1 kilodalton, and a 24.2 kilodalton fragment of said fibroblast growth factor, as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis.