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In vitro test for dermal corrosive properties

  • US 5,612,222 A
  • Filed: 01/11/1995
  • Issued: 03/18/1997
  • Est. Priority Date: 07/27/1992
  • Status: Expired due to Term
First Claim
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1. A method for determining the degree of corrosive toxicity of a test substance to human skin or membrane which method comprises:

  • (a) providing a biobarrier having a first face and a second face, which biobarrier mimics human skin or membrane, the test substance being applied to said first face, andwhich biobarrier is in contact at its second face with a detection system for said substance, wherein contact of said test substance with said detection system produces a detectable signal;

    orwhich biobarrier comprises at least one component which is broken down by a skin or membrane corrosive substance to form a breakdown product, said biobarrier being in contact at its second face with a breakdown product detection system wherein contact of said breakdown product with said detection system produces a detectable signal;

    (b) applying the test substance at a time T1 to said first face;

    (c) allowing the test substance to transit the biobarrier to the second face to contact the detection system and to produce said detectable signal;

    orallowing the test substance to react in transit with the biobarrier component to produce said breakdown product and allowing the breakdown product to transit to the second face of the biobarrier and to contact the breakdown product detection system and to produce a detectable signal;

    (d) detecting said signal at a subsequent time T2, said time T2 being that time at which said signal is first detectable at a predetermined level;

    (e) obtaining the time difference between said times T1 and T2; and

    (f) correlating said time difference with corrosive toxicity by comparing said time difference with time differences obtained using substances of known toxicity.

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