Optical method for the detection of cervical neoplasias using fluorescence spectroscopy
First Claim
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1. A method of detecting tissue abnormality in a tissue sample in vitro comprising:
- (i) providing a tissue sample;
(ii) sequentially illuminating said tissue sample in vitro with a set of at least two electromagnetic radiation wavelengths selected to cause said tissue sample to produce a set of fluorescence intensity spectra indicative of tissue abnormality;
(iii) detecting said set of fluorescence intensity spectra emitted from said tissue sample as a result of illumination with each of said wavelengths; and
(iv) calculating from said set of fluorescence intensity spectra, a probability that said tissue sample is normal or abnormal.
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Abstract
A method for detecting tissue abnormality, particularly precancerous cervical tissue, through fluorescence spectroscopy is disclosed. In vitro fluorescence measurements over a variety of different fluorescence spectra are used to screen tissue samples. Using a principal component analysis (PCA), it is possible to discriminate between normal and dysplastic tissues with relatively low false-positive and false-negative results.
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15 Claims
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1. A method of detecting tissue abnormality in a tissue sample in vitro comprising:
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(i) providing a tissue sample; (ii) sequentially illuminating said tissue sample in vitro with a set of at least two electromagnetic radiation wavelengths selected to cause said tissue sample to produce a set of fluorescence intensity spectra indicative of tissue abnormality; (iii) detecting said set of fluorescence intensity spectra emitted from said tissue sample as a result of illumination with each of said wavelengths; and (iv) calculating from said set of fluorescence intensity spectra, a probability that said tissue sample is normal or abnormal. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification