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DNA polymerase having modified nucleotide binding site for DNA sequencing

  • US 5,614,365 A
  • Filed: 11/10/1994
  • Issued: 03/25/1997
  • Est. Priority Date: 10/17/1994
  • Status: Expired due to Term
First Claim
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1. Modified gene encoding a modified Pol I-type DNA polymerase wherein said modified gene is modified to encode a tyrosine residue at an amino acid position corresponding to T7 DNA polymerase residue 526 or at an amino acid position corresponding to E. coli DNA polymerase residue 762 in its dNMP binding site to increase ability of said modified DNA polymerase to incorporate a dideoxynucleotide relative to a corresponding deoxynucleotide compared to the ability of a corresponding naturally-occurring unmodified DNA polymerase by at least 20-fold.

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