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Method and system for calibration of immunoassay systems through application of bayesian analysis

  • US 5,616,504 A
  • Filed: 01/09/1995
  • Issued: 04/01/1997
  • Est. Priority Date: 02/23/1993
  • Status: Expired due to Fees
First Claim
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1. A method for measuring a concentration of an analyte in a sample having an unknown concentration of the analyte using a binding assay in which a labelled binding partner for the analyte is allowed to react with the analyte to form a labeled complex in an assayed sample, wherein the labeled binding partner includes a tag which emits an experimental indicator and wherein the assayed sample emits in a predetermined period of time an amount of the experimental indicator, which amount is a response emitted by the assayed sample, the method comprising the steps of:

  • assaying a plurality of known samples using the binding assay, wherein each known sample has a known concentration of the analyte and is allowed to react with the labeled binding partner for the analyte to form a first labeled complex and the first labeled complex is detected to obtain a response emitted by each assayed known sample;

    assaying the unknown sample using the binding assay, wherein each unknown sample is allowed to react with the labeled binding partner for the analyte to form a second labeled complex and the second labeled complex is detected to obtain a response emitted by the assayed unknown sample;

    supplying a prior probability density for the unknown concentration and a probability model which specifics a standard curve relating an expected response to a concentration of the analyte;

    generating a posterior density for the unknown concentration based on the supplied prior density, the supplied probability model, and the responses for the assayed known samples and the assayed unknown sample, by applying Bayes'"'"' rule; and

    providing a characteristic of the posterior density as a measure of the concentration of the analyte in the unknown sample.

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