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Coding, promoter and regulator sequences of IRF-1

  • US 5,616,699 A
  • Filed: 11/18/1994
  • Issued: 04/01/1997
  • Est. Priority Date: 08/24/1988
  • Status: Expired due to Term
First Claim
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1. An isolated DNA molecule, wherein said molecule comprises a nucleic acid sequence, wherein said nucleic acid sequence:

  • (1) encodes an IRF-1 protein(a) that binds to a first recognition sequence (AAGTGA)4 ; and

    (b) that binds to a second recognition sequence at bases -64 to -100 of the human IFN-β

    gene;

    wherein said binding in steps (a) and (b) augments transcription of a coding sequence operably linked to a promoter that contains said first or second recognition sequence; and

    (2) wherein said nucleic acid sequence hybridizes to the antisense sequence of a DNA selected from the group consisting of the coding sequences;

    ##STR97## when the hybridization is performed at 65°

    degrees for 20 hours in a medium consisting essentially of 1M NaCl, 50 mM Tris-HCl, pH 7.4, 10 mM EDTA, 0.1% sodium dodecyl sulfate, 0.2% ficoll, 0.2% polyvinylpyrrolidone, 0.2% bovine serum albumin, 50 μ

    g/ml E. coli DNA, said nucleic acid sequence and said antisense sequence.

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