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Bioluminescence measurement system

  • US 5,618,682 A
  • Filed: 02/08/1994
  • Issued: 04/08/1997
  • Est. Priority Date: 02/10/1993
  • Status: Expired due to Term
First Claim
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1. A method for detecting the presence of luciferase in a biological sample by measuring the luminescence of said sample comprising:

  • (a) mixing a sample suspected of containing luciferase with a reaction mixture containing luciferin, adenosine triphosphate ("ATP"), cofactors necessary for luciferase catalytic activity, and adenosine monophosphate ("AMP"), the amounts of ingredients in said reaction mixture being selected to produce luminescence having a duration of at least one hour and an intensity that varies substantially linearly with time; and

    (b) measuring said luminescence produced by said reaction mixture containing said sample.

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