BMP-2 products
First Claim
Patent Images
1. A purified protein produced by the steps of(a) culturing in a suitable culture medium a cell transformed with an expression vector comprising a DNA as shown in FIGS. 2A-C or a degenerative sequence thereof and which encodes a protein containing the amino acid sequence ##STR1## and (b) recovering said protein from said culture medium.
1 Assignment
0 Petitions
Accused Products
Abstract
Purified BMP-2 proteins and processes for producing them are disclosed. They may be used in the treatment of bone and cartilage defects and in wound healing and related tissue repair.
-
Citations
10 Claims
-
1. A purified protein produced by the steps of
(a) culturing in a suitable culture medium a cell transformed with an expression vector comprising a DNA as shown in FIGS. 2A-C or a degenerative sequence thereof and which encodes a protein containing the amino acid sequence ##STR1## and (b) recovering said protein from said culture medium.
-
2. A purified protein produced by the steps of
(a) culturing in a suitable culture medium a cell transformed with an expression vector comprising an allelic variant of the DNA sequence as shown in FIG. 2A-C and which encodes a BMP-2A protein; - and
(b) recovering said protein from said culture medium.
- and
-
3. A purified protein produced by the steps of
(a) culturing in a suitable culture medium a cell transformed with an expression vector comprising a DNA sequence which hybridizes under stringent hybridization conditions to the DNA sequence as shown in FIG. 2A-C and encodes a protein containing the amino acid sequence ##STR2## and (b) recovering said protein from said culture medium.
-
5. A purified protein comprising the amino acid sequence:
- ##STR3##
-
6. A purified protein produced by the steps of
(a) culturing in a suitable culture medium a cell transformed with an expression vector comprising a DNA as shown in FIG. 3A-C or a degenerative sequence thereof and which encodes a protein containing the amino acid sequence ##STR4## and (b) recovering said protein from said culture medium.
-
7. A purified protein produced by the steps of
(a) culturing in a suitable culture medium a cell transformed with an expression vector comprising an allelic sequence of the DNA as shown in FIG. 3A-C and which encodes a BMP-2B protein; - and
(b) recovering said protein from said culture medium.
- and
-
8. A purified protein produced by the steps of
(a) culturing in a suitable culture medium a cell transformed with an expression vector comprising a DNA which hybridizes under stringent hybridization conditions to the DNA sequence as shown in FIG. 3A-C and encodes a protein containing the amino acid sequence ##STR5## and (b) recovering said protein from said culture medium.
-
10. A purified protein comprising the amino acid sequence:
- ##STR6##
Specification