BMP-2 products

• US 5,618,924 A
• Filed: 03/18/1991
• Issued: 04/08/1997
• Est. Priority Date: 07/01/1986
• Status: Expired due to Term

• Current Assignee: Genetics Institute LLC (Pfizer Inc.)
• Original Assignee: Genetics Institute LLC (Pfizer Inc.)
• Inventors: Wozney, John M., Rosen, Vicki, Wang, Elizabeth A.
• Primary Examiner(s): Allen, Marianne P.
• Application Number:
  US07/655,579
• Time in Patent Office: 2,213 Days
• Field of Search: 530/399, 530/350, 530/324, 435/69.1, 435/69.4, 536/27, 514/12
• US Class Current: 530/399
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61L 24/043 Mixtures of macromolecular materials
• CPC Class Codes: A61L 27/227 Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
• CPC Class Codes: C07K 14/51 Bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
• CPC Class Codes: C08L 89/00 Compositions of proteins; Compositions of derivatives thereof foodstuff preparations A23J3/00

Abstract

Purified BMP-2 proteins and processes for producing them are disclosed. They may be used in the treatment of bone and cartilage defects and in wound healing and related tissue repair.

Claims

1. A purified protein produced by the steps of

(a) culturing in a suitable culture medium a cell transformed with an expression vector comprising a DNA as shown in FIGS. 2A-C or a degenerative sequence thereof and which encodes a protein containing the amino acid sequence ##STR1## and (b) recovering said protein from said culture medium.

4. The protein of any one of claims 1, 2, and 3 wherein the cell is mammalian.

2. A purified protein produced by the steps of

(a) culturing in a suitable culture medium a cell transformed with an expression vector comprising an allelic variant of the DNA sequence as shown in FIG. 2A-C and which encodes a BMP-2A protein;

and

(b) recovering said protein from said culture medium.

3. A purified protein produced by the steps of

(a) culturing in a suitable culture medium a cell transformed with an expression vector comprising a DNA sequence which hybridizes under stringent hybridization conditions to the DNA sequence as shown in FIG. 2A-C and encodes a protein containing the amino acid sequence ##STR2## and (b) recovering said protein from said culture medium.

5. A purified protein comprising the amino acid sequence:

##STR3##

6. A purified protein produced by the steps of

(a) culturing in a suitable culture medium a cell transformed with an expression vector comprising a DNA as shown in FIG. 3A-C or a degenerative sequence thereof and which encodes a protein containing the amino acid sequence ##STR4## and (b) recovering said protein from said culture medium.

9. The protein of any one of claims 6, 7, and 8 wherein said cell is mammalian.

7. A purified protein produced by the steps of

(a) culturing in a suitable culture medium a cell transformed with an expression vector comprising an allelic sequence of the DNA as shown in FIG. 3A-C and which encodes a BMP-2B protein;

and

(b) recovering said protein from said culture medium.

8. A purified protein produced by the steps of

(a) culturing in a suitable culture medium a cell transformed with an expression vector comprising a DNA which hybridizes under stringent hybridization conditions to the DNA sequence as shown in FIG. 3A-C and encodes a protein containing the amino acid sequence ##STR5## and (b) recovering said protein from said culture medium.

10. A purified protein comprising the amino acid sequence:

##STR6##