Apparatus for reduction of bias in amperometric sensors

• US 5,620,579 A
• Filed: 05/05/1995
• Issued: 04/15/1997
• Est. Priority Date: 05/05/1995
• Status: Expired due to Term

• Current Assignee: Bayer Corporation (Bayer AG)
• Original Assignee: Bayer Corporation (Bayer AG)
• Inventors: Genshaw, Marvin A., Yip, Kin F., Huang, Dijia, Musho, Matthew K.
• Primary Examiner(s): Tung, T.
• Application Number:
  US08/435,993
• Time in Patent Office: 711 Days
• Field of Search: 204/402, 204/403, 204/406, 205/775, 205/777.5
• US Class Current: 204/402
• CPC Class Codes: C12Q 1/004 mediator-assisted

Abstract

Apparatus and method are provided for determining the concentration of an analyte in a fluid test sample by applying the fluid test sample to the surface of a working electrode which is electrochemically connected to a reference electrode which surface bears a composition comprising an enzyme specific for the analyte. A mediator is reduced in response to a reaction between the analyte and the enzyme. An oxidizing potential is applied between the electrodes to return at least a portion of the mediator back to its oxidized form before determining the concentration of the analyte to thereby increase the accuracy of the analyte determination. Following this initially applied potential, the circuit is switched to an open circuit or to a potential that substantially reduces the current to minimize the rate of electrochemical potential at the working electrode. A second potential is applied between the electrodes and the current generated in the fluid test sample is measured to determine analyte concentration. Optionally, the accuracy of the analyte determination is further enhanced algorithmically.

Claims

1. Apparatus for analyte determination in a test sample comprising:

amperometric sensor means for receiving the test sample;

timer means for identifying a burnoff time interval, a delay time interval, and a read time interval;

said read time interval following said delay time interval;

voltage potential means responsive to said timer means for applying a first voltage potential which is a burnoff voltage potential to said amperometric sensor means during said burnoff time interval and for applying a second voltage potential which is a read voltage potential to said amperometric sensor means during said read time interval;

said burnoff voltage potential and said read voltage potential having a common polarity;

means responsive to said timer means and said voltage potential means for measuring a first current i₁ resulting from said applied burnoff voltage potential and a second current i₂ resulting from said applied read voltage potential; and

means responsive to said current measuring means for determining an analyte value of the test sample.

2. Apparatus of claim 1 further includes means for storing predetermined characteristic parameter values relating to said amperometric sensor means and means responsive to said first current i₁ and second current i₂ measuring means for calculating a bias correction value.

3. Apparatus of claim 2 wherein said analyte value determining means is responsive to said bias correction value calculating means for determining said analyte value.

4. Apparatus of claim 3 wherein said calculated bias correction value is defined by means for calculating:

##EQU8## where i₁ represents said first current resulting from said burnoff voltage potential, i₂ represents said second current resulting from said read voltage potential and Int, slope, i_1-lo, i_2-lo and s₁ represent said predetermined characteristic parameter values relating to said amperometric sensor means, Int being the intercept of current i₂, slope being the slope of current i₂, i_1-lo being the average current i₁ at the low analyte calibration level, i_2-lo being the average current i₂ at the low analyte calibration level and s₁ being the slope current i₁.

5. Apparatus of claim 4 wherein said analyte is glucose and said determined analyte value is represented by:

##EQU9## where k represents a selected bias scaling factor having a value between 0 and 1.