Causative agent of the mystery swine disease, vaccine compositions and diagnostic kits

• US 5,620,691 A
• Filed: 11/26/1993
• Issued: 04/15/1997
• Est. Priority Date: 06/06/1991
• Status: Expired due to Term

• Current Assignee: Stichting Dienst Landbouwkundig Onderzoek
• Original Assignee: Stichting Centraal Diergeneeskundig Instituut
• Inventors: Pol, Joannes M. A., Terpstra, Catharinus, Wensvoort, Gert
• Primary Examiner(s): Housel, James C.
• Assistant Examiner(s): Minnifield, N. M.
• Application Number:
  US08/157,005
• Time in Patent Office: 1,236 Days
• Field of Search: 424/218.1, 424/221.1, 424/204.1, 424/815, 424/184.1
• US Class Current: 424/184.1
• CPC Class Codes: A61K 2039/525 Virus
• CPC Class Codes: A61K 2039/5252 inactivated (killed)
• CPC Class Codes: A61K 2039/5254 avirulent or attenuated
• CPC Class Codes: A61K 2039/53 DNA (RNA) vaccination
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: A61K 39/12 Viral antigens
• CPC Class Codes: A61P 31/12 Antivirals
• CPC Class Codes: A61P 31/14 for RNA viruses
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C07K 16/10 from RNA viruses
• CPC Class Codes: C12N 2770/10021 Viruses as such, e.g. new isolates, mutants or their genomic sequences
• CPC Class Codes: C12N 2770/10022 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2770/10034 Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
• CPC Class Codes: C12N 7/00 Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 1/6888 for detection or identification of organisms
• CPC Class Codes: Y10S 424/815 Viral vaccine for porcine species, e.g. swine
• CPC Class Codes: Y10S 435/975 Kit

Abstract

Composition of matter comprising the causative agent of Mystery Swine Disease, Lelystad Agent, in a live, attenuated, dead, or recombinant form, or a part or component of it. Vaccine compositions and diagnostic kits based thereon. Recombinant nucleic acid comprising a Lelystad Agent-specific nucleotide sequence. Peptides comprising a Lelystad Agent-specific amino acid sequence. Lelystad Agent-specific antibodies.

Claims

1. An isolated causative agent of Mystery Swine Disease identified as deposit number I-1102, deposited Jun. 5, 1991 with the Institut Pasteur, Paris, France.

2. A composition of matter comprising virus identified as Institut Pasteur deposit number I-1102.

3. A composition of matter in accordance with claim 2 wherein said virus is killed.

4. A vaccine composition comprising a virus having all the attributes of Lelystad Agent deposited Jun. 5, 1991 with the Institut Pasteur, Paris, France, deposit number I-1102, and a suitable carrier.

5. A vaccine composition in accordance with claim 4 wherein the virus is killed.

6. The vaccine composition of claim 4 further comprising an adjuvant.

7. The vaccine composition of claim 5 further comprising an adjuvant.

8. Isolated Lelystad Agent comprising a virus, said virus characterized in being sensitive to chloroform and having a size smaller than 200 nanometers, said virus further being immunoreactive with serum antibodies of a sow, said serum antibodies obtained by:

a) intranasally inoculating the sow with two milliliters of the virus identified as deposit number I-1102, deposited Jun. 5, 1991 with the Institut Pasteur, Paris, France (at passage level 3, titer 10⁴.8 TCID₅₀ /milliliter); and

b) collecting serum antibodies from the thus inoculated sow after 25 to 33 days.

9. The isolated Lelystad Agent of claim 8 wherein said virus has been inactivated.

10. The isolated Lelystad Agent of claim 9 in combination with an adjuvant.