Molecular recognition at surfaces derivatized with self-assembled monolayers
First Claim
1. A species having a formula:
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space="preserve" listing-type="equation">X--R--Ch--Mwherein X represents a functional group and R represents a spacer moiety that, together, are able to promote formation from solution of a self-assembled monolayer on a surface, Ch represents a chelating agent that coordinates a metal ion, and M represents a metal ion coordinated to the chelating agent, wherein M also is coordinated to a biological binding partner of a biological molecule via coordination sites not filled by the chelating agent or includes coordination sites not filled by the chelating agent that, upon exposure to a polyamino acid tag, are able to become coordinated by the polyamino acid tag.
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Accused Products
Abstract
An article suitable for use as a biosensor includes a species of a formula X--R--Ch adhered to a surface of the article as part of a self-assembled monolayer. X is a functionality that adheres to the surface, R is a spacer moiety, and Ch is a chelating agent. A metal ion can be coordinated by the chelating agent, and a polyamino acid-tagged biological binding partner of a target biological molecule coordinated to the metal ion. A method of the invention involves bringing the article into contact with a medium containing or suspected of containing the target biological molecule and allowing the biological molecule to biologically bind to the binding partner. The article is useful particularly as a surface plasmon resonance chip.
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Citations
28 Claims
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1. A species having a formula:
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space="preserve" listing-type="equation">X--R--Ch--Mwherein X represents a functional group and R represents a spacer moiety that, together, are able to promote formation from solution of a self-assembled monolayer on a surface, Ch represents a chelating agent that coordinates a metal ion, and M represents a metal ion coordinated to the chelating agent, wherein M also is coordinated to a biological binding partner of a biological molecule via coordination sites not filled by the chelating agent or includes coordination sites not filled by the chelating agent that, upon exposure to a polyamino acid tag, are able to become coordinated by the polyamino acid tag. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A species having a formula:
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space="preserve" listing-type="equation">X--R--Ch--Mwherein X represents a functional group and R represents a spacer moiety that, together, are able to promote formation from solution of a self-assembled monolayer on a gold surface, Ch represents a chelating agent that coordinates a metal ion, selected from the group consisting of bidentate, tridentate, and quadradentate chelating agents, and M represents a metal ion coordinated to the chelating agent, wherein M also is coordinated to a biological binding partner of a biological molecule via coordination sites not filled by the chelating agent or includes coordination sites not filled by the chelating agent that, upon exposure to a polyamino acid tag, are able to become coordinated by the polyamino acid tag. - View Dependent Claims (15, 16, 17, 18, 19, 20)
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21. A species having a formula:
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space="preserve" listing-type="equation">X--R--Ch--M--BPwherein X represents a functional group and R represents a spacer moiety that, together, are able to promote formation from solution of a self-assembled monolayer on a surface, Ch represents a chelating agent that coordinates a metal ion, M represents a metal ion coordinated to the chelating agent, and BP represents a biological binding partner of a biological molecule coordinated to the metal ion. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28)
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Specification