Imaging system transfer function control method and apparatus

• US 5,621,519 A
• Filed: 07/31/1995
• Issued: 04/15/1997
• Est. Priority Date: 07/31/1995
• Status: Expired due to Term

• Current Assignee: Tripath Imaging Incorporated (Becton, Dickinson & Co)
• Original Assignee: NeoPath, Inc. (Becton, Dickinson & Co)
• Inventors: Hayenga, Jon W., Oh, Seho, Hansen, Kim J., Frost, Keith L.
• Primary Examiner(s): Evans, F. L.
• Application Number:
  US08/509,182
• Time in Patent Office: 624 Days
• Field of Search: 356/124.5
• US Class Current: 356/124.5
• CPC Class Codes: G01M 11/0292 of objectives by measuring the optical modulation transfer function photometry G01J
• CPC Class Codes: H04N 17/002 for television cameras

Abstract

Accurately measuring and controlling the system transfer function of an imaging system, by eliminating interference from aliases. An image is captured of an image primitive with a uniaxial pattern, oriented at an angle to the primary axes of a sampling array. In order to accurately measure the system transfer function, a two-dimensional frequency representation is computed. The aliased components of the representation are separated from the unaliased components by translation in frequency along the axis perpendicular to the axis of the image primitive. Accurate measurement of the system transfer function makes it possible to accurately control the system transfer function.

Claims

1. A method to measure a system transfer function of an imaging system having an input and an image sampler with a discrete image output wherein the discrete image output can be represented by a frequency representation with aliased components and unaliased components, the method comprising the steps of:

(a) providing an image primitive to the input, the image primitive comprising a substantially uniaxial pattern having a predetermined frequency representation, the image primitive being oriented at a predetermined angle relative to the image sampler wherein the predetermined angle is chosen to separate the aliased components from the unaliased components;

(b) obtaining a discrete image of the image primitive with the imaging system;

(c) computing a two-dimensional frequency representation of the discrete image;

(d) selecting a region of the two-dimensional frequency representation; and

(e) comparing the region to the predetermined frequency representation to compute the system transfer function.

2. The method of claim 1 wherein the image primitive comprises a bar pattern.

3. The method of claim 1 wherein the substantially uniaxial pattern further comprises a substantially periodic pattern.

4. The method of claim 1 wherein the predetermined angle is a small angle.

5. The method of claim 1 wherein the predetermined angle is in a range of greater than 0°

and less than 15°

.

6. The method of claim 1 further comprising the step of multiplying the discrete image by a windowing function.

7. The method of claim 1 further comprising the steps of filtering with a filter and subsampling the discrete image in a predetermined axis to suppress noise from the discrete image.

8. The method of claim 7 further comprising the step of correcting an effect of filtering by dividing a frequency representation of the discrete image by a frequency representation of the filter.

9. The method of claim 1, wherein the system transfer function has an STF phase, wherein the region has a signal phase, and wherein the predetermined frequency representation has a pattern phase further comprising the steps of:

(a) subtracting the pattern phase from the signal phase to generate a difference phase;

(b) determining a linear phase that best fits the difference phase; and

(c) computing the STF phase as a difference between the linear phase and the difference phase.

10. The method of claim 9 wherein the step of determining a linear phase that best fits the difference phase further comprises setting a phase of a fundamental frequency of the difference phase to zero.

11. The method of claim 3, wherein the substantially periodic pattern generates frequencies with frequency peaks, further comprises the steps of:

(a) locating of the frequency peaks; and

(b) measuring their amplitudes and phases.

12. The method of claim 11 further comprising the step of fitting the frequency peaks using a model peak comprising a scaled and translated frequency representation of a windowing function.

13. The method of claim 12 further comprising the step of applying minimization techniques to find values of an amplitude, phase, and location of the model peak which minimizes a summed square difference between the model peak and data.

14. The method of claim 13 wherein the step of applying minimization techniques further comprises applying Newton'"'"'s minimization method.

15. The method of claim 11 wherein a magnitude of a system transfer function is computed by dividing the magnitude of each frequency peak by the magnitude of a corresponding peak of the predetermined frequency representation.

16. The method of claim 1 wherein the frequency representations comprise Fourier Transforms.

17. The method of claim 1 wherein the step of computing a two-dimensional frequency representation of the discrete image further comprises computing a Fast Fourier Transform.

18. The method of claim 1 where the step of selecting a region of the two-dimensional frequency representation further comprises determining a band passing through an origin of the frequency representation oriented at an angle chosen to maximize power within the band.

19. The method of claim 1 where the step of selecting a region of the two-dimensional frequency representation further comprises selecting a band corresponding to the predetermined angle.

20. The method of claim 12 further comprising the step of applying minimization techniques to find values of the amplitude phase and location of multiple model peaks which minimize the sum square difference between the sum of the model peaks and the data.

21. An apparatus to measure frequency response of an imaging system having an input and an image sampler with a discrete image output wherein the discrete image output can be represented by a frequency representation with aliased components and unaliased components, the apparatus comprising:

(a) an image primitive placed in view of the input, the image primitive comprising a substantially uniaxial pattern having a predetermined frequency representation, the image primitive being oriented at a predetermined angle relative to the image sampler wherein the predetermined angle is chosen to separate the aliased components from the unaliased components;

(b) means, connected to the discrete image output, for computing a two-dimensional frequency representation of the discrete image output, having a computing output;

(c) means, connected to the computing output, for selecting a region of the two-dimensional frequency representation, having a selected region output; and

(d) means, connected to the selected region output, for comparing the region to the predetermined frequency representation to compute an optical transfer function, having an optical transfer function output.

22. The apparatus of claim 21 wherein the means for computing a two dimensional frequency representation of the discrete image output further comprises a digital computer.

23. The apparatus of claim 21 wherein the means for selecting a region of the two-dimensional frequency representation further comprises a digital computer.

24. The apparatus of claim 21 wherein the means for comparing the region to the predetermined frequency representation to compute the optical transfer function output further comprises a digital computer.

25. The apparatus of claim 21 wherein the image sampler further comprises an electronic camera.

26. The apparatus of claim 21 wherein the image sampler further comprises a charge coupled device camera.

27. An imaging system control apparatus, for an imaging system having a transfer function control input, wherein the imaging system has a system transfer function and system transfer function control input, the imaging system control apparatus comprising:

(a) means for measuring the system transfer function of the imaging system having a system transfer measure output; and

(b) means for controlling the system transfer function in response to the system transfer measure output connected to the system transfer measure output, wherein the means for controlling the system transfer function is connected to the system transfer function control input.

28. The apparatus of claim 27 wherein the imaging system further comprises a charge coupled device camera.

29. The apparatus of claim 27 wherein the means for controlling the system transfer function in response to the system transfer measure output comprises a plurality of trim resistor - capacitor combinations.

30. The apparatus of claim 27 wherein the means for controlling the system transfer function in response to the system transfer measure output comprises a digital filter.

31. The apparatus of claim 27 wherein the imaging system further comprises an image interpretation apparatus imaging system.

32. A method of modifying a system transfer function of an image system comprising the steps of:

(a) measuring an original system transfer function at multiple focal planes;

(b) selecting a system transfer function with a highest modulation transfer function;

(c) checking if the system transfer function is within predetermined limits;

(d) calculating imaging system component values to optimize system transfer function;

(e) checking if a modeled system transfer function is within the predetermined limits;

(f) modifying the image system using the calculated components;

(g) measuring a modified system transfer function at multiple focal planes;

(h) selecting the system transfer function with the highest modulation transfer function; and

(i) checking if the system transfer function is within the predetermined limits.