High-affinity oligonucleotide ligands to secretory phospholipase A2 (sPLA.sub.2)
First Claim
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1. A method for identifying nucleic acid ligands and nucleic acid ligand sequences to secretory phospholipase A2 (sPLA2) comprising:
- a) contacting a candidate mixture of nucleic acids with sPLA2, wherein nucleic acids having an increased affinity to the sPLA2 relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;
b) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture; and
c) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acid sequences with relatively higher affinity and specificity for binding to sPLA2, whereby nucleic acid ligands and nucleic acid ligand sequences of sPLA2 may be identified.
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Abstract
This invention discloses high-affinity oligonucleotide ligands to human secretory phospholipase A2 (sPLA2), specifically RNA ligands having the ability to bind to sPLA2, and the methods for obtaining such ligands.
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Citations
11 Claims
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1. A method for identifying nucleic acid ligands and nucleic acid ligand sequences to secretory phospholipase A2 (sPLA2) comprising:
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a) contacting a candidate mixture of nucleic acids with sPLA2, wherein nucleic acids having an increased affinity to the sPLA2 relative to the candidate mixture may be partitioned from the remainder of the candidate mixture; b) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture; and c) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acid sequences with relatively higher affinity and specificity for binding to sPLA2, whereby nucleic acid ligands and nucleic acid ligand sequences of sPLA2 may be identified. - View Dependent Claims (2, 3, 4, 5, 6)
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- 7. A purified and isolated non-naturally occurring nucleic acid ligand to secretory phospholipase A2 (sPLA2).
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10. A purified and isolated non-naturally occuring RNA ligand to sPLA2 comprising the sequences selected from the group consisting of SEQ ID NOS:
- 97-100.
Specification