Electrically hydrolyzed salines as microbiocides for in vitro treatment of contaminated fluids containing blood

• US 5,622,848 A
• Filed: 07/15/1994
• Issued: 04/22/1997
• Est. Priority Date: 05/23/1990
• Status: Expired due to Fees

• Current Assignee: Medical Discoveries, Inc. (Global Clean Energy Holdings Inc.)
• Original Assignee: Medical Discoveries, Inc. (Global Clean Energy Holdings Inc.)
• Inventors: Morrow, Robert E.
• Primary Examiner(s): Rollins, John W.
• Assistant Examiner(s): Ware, Deborah K.
• Application Number:
  US08/275,904
• Time in Patent Office: 1,012 Days
• Field of Search: 435/173.1, 435/173.9, 435/817, 424/613, 424/661, 204/228
• US Class Current: 435/173.1
• CPC Class Codes: A23L 29/37 Sugar alcohols
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 31/165 having aromatic rings, e.g. colchicine, atenolol, progabide
• CPC Class Codes: A61K 31/375 Ascorbic acid, i.e. vitamin C; Salts thereof
• CPC Class Codes: A61K 31/715 Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
• CPC Class Codes: A61K 33/00 Medicinal preparations containing inorganic active ingredients
• CPC Class Codes: A61K 33/14 Alkali metal chlorides; Alkaline earth metal chlorides
• CPC Class Codes: A61K 33/20 Elemental chlorine; Inorganic compounds releasing chlorine
• CPC Class Codes: A61K 33/40 Peroxides
• CPC Class Codes: A61K 33/42 Phosphorus; Compounds thereof
• CPC Class Codes: A61K 35/08 Mineral waters; Sea water
• CPC Class Codes: A61K 38/063 Glutathione
• CPC Class Codes: A61K 38/44 Oxidoreductases (1)
• CPC Class Codes: A61K 38/446 Superoxide dismutase (1.15)
• CPC Class Codes: A61K 8/345 containing more than one hydroxy group
• CPC Class Codes: A61K 8/60 Sugars; Derivatives thereof
• CPC Class Codes: A61K 8/73 Polysaccharides
• CPC Class Codes: A61K 8/732 Starch; Amylose; Amylopectin; Derivatives thereof
• CPC Class Codes: A61L 2/0088 Liquid substances
• CPC Class Codes: A61P 31/04 Antibacterial agents
• CPC Class Codes: A61P 31/10 : Antimycotics
• CPC Class Codes: A61P 31/12 : Antivirals
• CPC Class Codes: A61P 31/14 : for RNA viruses
• CPC Class Codes: A61P 31/16 : for influenza or rhinoviruses
• CPC Class Codes: A61P 31/20 : for DNA viruses
• CPC Class Codes: A61P 31/22 : for herpes viruses
• CPC Class Codes: A61P 33/00 : Antiparasitic agents
• CPC Class Codes: A61P 37/04 : Immunostimulants
• CPC Class Codes: A61Q 11/00 : Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
• CPC Class Codes: A61Q 19/00 : Preparations for care of the skin
• CPC Class Codes: C02F 1/4618 : for producing "ionised" acidic or basic water
• CPC Class Codes: C02F 1/4672 : by electrooxydation
• CPC Class Codes: C02F 1/4674 : with halogen or compound of halogens, e.g. chlorine, bromine
• CPC Class Codes: C02F 2103/026 : Treating water for medical or cosmetic purposes
• CPC Class Codes: C02F 2303/04 : Disinfection
• CPC Class Codes: C07C 31/18 : Polyhydroxylic acyclic alcohols
• CPC Class Codes: C12N 2740/16063 : by chemical treatment
• CPC Class Codes: C12N 7/00 : Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00
• CPC Class Codes: Y02A 50/30 : Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
• CPC Class Codes: Y10S 435/817 : Enzyme or microbe electrode

Abstract

A microbiocidal solution for in vivo and in vitro treatment of microbial infections includes an electrolyzed saline containing regulated amounts of ozone and active chlorine species wherein the ozone content is between about 5 and 100 mg/L and the active chlorine species content of between about 5 and 300 ppm. The active chlorine species comprises free chlorine, hypochlorous acid and the hypochlorite ion as measured by a chlorine selective electrode. The solution is prepared by subjecting a 1% or less saline to electrolysis under conditions sufficient to produce the desired active ingredients. The solution is preferably utilized at an isotonic saline concentration and may be adjusted with hypertonic saline. The solution may be used for the in vitro treatment of infected whole blood, blood cells or plasma to reduce contamination and is effective in treatment of fluids infected with HIV, hepatitis and other viral, bacterial and fungal agents. The solution may also be administered to warm blooded animals, including humans, by intravenous injection or other modes for similar purposes. If desired neutralizing agents, such as antioxidants, may be administered in correlation with the solution.

Claims

1. A method for the in vitro treatment of a microbially contaminated fluid selected from the group consisting of whole blood, blood cells, blood plasma, and mixtures thereof which comprises contacting said fluid with an effective amount of a microbiocidal solution comprising an electrolyzed saline containing ozone and active chlorine species wherein the ozone content is in the range of about 5 and 100 mg/L and the active chlorine species content is in the range of about 5 and 300 ppm for a time sufficient to reduce the microbial contamination in said fluid while maintaining therapeutic characteristics thereof.

2. The method of claim 1 wherein said microbially contaminated fluid is contaminated by an agent selected from the group consisting of Epstein-Barr virus, hepatitis A, B or C virus, rhinovirus, rubeola virus, rubella virus, parvovirus, papilloma virus, influenza viruses, parainfluenza viruses, enteroviruses, Herpes simplex viruses, Varicella-zoster viruses, Adenoviruses, respiratory syncytial viruses, alphaviruses, flaviviruses, retroviruses, and agents of bacteremia, septicemia, fungal infections, parasitic infections, mycobacterial infections, Bacterial Gram positive infections, Bacterial Gram negative infections, superficial and systemic infections.

3. The method of claim 2 wherein said microbiocidal solution is an isotonic saline solution.

4. The method of claim 3 wherein said microbially contaminated fluid is whole blood.

5. The method of claim 3 wherein said microbially contaminated fluid is blood cells.

6. The method of claim 3 wherein said microbially contaminated fluid is blood plasma.

7. The method of claim 3 wherein said microbially contaminated fluid is retrovirus contaminated.

8. The method of claim 7 wherein said retrovirus is HIV.

9. The method of claim 8 wherein said microbially contaminated fluid is whole blood.

10. The method of claim 8 wherein said microbially contaminated fluid is blood cells.

11. The method of claim 8 wherein said microbially contaminated fluid is blood plasma.

12. The method of claim 3 wherein said microbially contaminated fluid is contaminated by a hepatitis virus.