Analyte detection through observed optical modulation of polymerized lipid layers

• US 5,622,872 A
• Filed: 05/11/1995
• Issued: 04/22/1997
• Est. Priority Date: 06/15/1989
• Status: Expired due to Fees

• Current Assignee: Biocircuits Corporation
• Original Assignee: Biocircuits Corporation
• Inventors: Ribi, Hans O.
• Primary Examiner(s): Spiegel, Carol A.
• Application Number:
  US08/439,413
• Time in Patent Office: 712 Days
• Field of Search: 435/4, 435/7.1, 435/14, 435/291, 435/288, 435/808, 435/817, 435/7.5, 435/288.7, 427/2.11, 436/518, 436/524, 436/528, 436/529, 436/531, 436/71, 436/149, 436/150, 436/532, 204/403, 356/352, 422/57-58, 422/82.06-82.09, 422/82.11
• US Class Current: 436/518
• CPC Class Codes: B01J 2219/00734 Lipids
• CPC Class Codes: G01N 33/542 with steric inhibition or signal modification, e.g. fluorescent quenching
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property

Abstract

Bioelectronic sensors are provided employing a thin surfactant polymeric electrically conducting layer to which may be bound members of specific binding pairs. Binding of an analyte or a reagent to the specific binding pair member layer may change the electrical, optical, or structural properties of the layer for measurement of analyte. The change in the polymeric layer provides for a sensitive measurement.

Claims

1. A method for detecting the presence of an analyte in a sample, wherein said analyte is a ligand or a receptor of a specific binding pair, said method comprising:

contacting said sample with a polymerized surfactant layer having optical properties, polymerized from polyunsaturated surfactant monomers of the formula;

space="preserve" listing-type="equation">(A).sub.a (D).sub.a C.sub.x (C.tbd.C).sub.n C.sub.y LB

wherein;

A is a functionality for linking to an underlying substrate;

a is 0 or 1;

C is carbon;

x and y are at least 1 and the sum of x and y is in the range of 4-32;

n is 2;

D and L are bond or linking groups; and

B is a specific binding member which binds to said receptor;

with one terminus of each of said monomers proximal to the underlying substrate and the other terminus comprising B, said polymerization occurring at the C.tbd.C groups of said monomers;

wherein said optical properties of said surfactant layer change due to specific binding of said receptor to B, with the proviso that when said analyte is said ligand aid sample comprises a predetermined amount of said receptor;

detecting said change in said optical properties upon specific binding of said receptor to B; and

relating said change to the presence of said analyte in said sample;

whereby the presence of said analyte in said sample is detected.

2. The method according to claim 1, wherein said change in said optical properties of said surfactant layer is a change in absorbance properties of said surfactant layer.

3. The method according to claim 1, wherein said change in said optical properties of said surfactant layer is a change in fluorescence emission properties of said surfactant layer.

4. A method for detecting the presence of an analyte in a sample, wherein said analyte is a ligand or a receptor of a specific binding pair, said method comprising:

contacting said sample with a polymerized surfactant layer, polymerized from polyunsaturated surfactant monomers of the formula;

space="preserve" listing-type="equation">(A).sub.a (D).sub.a C.sub.x (C.tbd.C).sub.n C.sub.y LB

wherein;

A is a functionality for linking to an underlying substrate;

a is 0 or 1;

C is carbon;

x and y are at least 1 and the sum of x and y is in the range of 4-32;

n is 2;

D and L are bond or linking groups; and

B is a specific binding member which binds to said receptor;

with one terminus of each of said monomers proximal to the underlying substrate and the other terminus comprising B, said polymerization occurring at the C.tbd.C groups of said monomers;

wherein absorbance properties of said surfactant layer change in response to conformational changes of said surfactant layer due to specific binding of said receptor to B, with the proviso that when said analyte is said ligand said sample comprises a predetermined amount of said receptor;

comparing said absorbance properties between said sample-contacted surfactant layer with absorbance properties of a control; and

relating any difference in absorbance properties between said sample-contacted surfactant layer and said control to the presence of said analyte in said sample;

whereby the presence of said analyte in said sample is detected.

5. A method for detecting the presence of an analyte in a sample, wherein said analyte is a ligand or a receptor of a specific binding pair, said method comprising:

contacting said sample with a polymerized surfactant layer, polymerized from polyunsaturated surfactant monomers of the formula;

space="preserve" listing-type="equation">(A).sub.a (D).sub.a C.sub.x (C.tbd.C).sub.n C.sub.y LB

wherein;

A is a functionality for linking to an underlying substrate;

a is 0 or 1;

C is carbon;

x and y are at least 1 and the sum of x and y is in the range of 4-32;

n is 2;

D and L are bond or linking groups; and

B is a specific binding member which binds to said receptor;

with one terminus of each of said monomers proximal to the underlying substrate and the other terminus comprising B, said polymerization occurring at the C.tbd.C groups of said monomers;

wherein fluorescence emission properties of said surfactant layer change due to specific binding of said receptor to B, with the proviso that when said analyte is said ligand said sample comprises a predetermined amount of said receptor;

comparing said fluorescence emission properties between said sample-contacted surfactant layer with fluorescent emission properties of a control; and

relating any difference in fluorescence emission properties between said sample-contacted surfactant layer and said control to the presence of said analyte in said sample;

whereby the presence of said analyte in said sample is detected.