Culture medium and method for culturing body fluids containing antibiotics

• US 5,624,814 A
• Filed: 10/19/1994
• Issued: 04/29/1997
• Est. Priority Date: 08/20/1981
• Status: Expired due to Fees

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Broman, Rodney, Waters, John R.
• Primary Examiner(s): Scheiner, Toni R.
• Application Number:
  US08/326,155
• Time in Patent Office: 923 Days
• Field of Search: 435/29, 435/31, 435/32, 435/33, 435/34, 435/36, 435/38, 435/244, 435/253.6, 435/261
• US Class Current: 435/29
• CPC Class Codes: C12Q 1/045 Culture media therefor

Abstract

A nutrient culture medium which isolates antibiotics and other microbial growth inhibitors during culturing of a microorganism is shown. The culture medium includes an aqueous dispersion of nutrient materials and an effective amount of an isolating substance or substances capable of isolating antimicrobial materials during culturing of a microorganism.

The isolating substances are selected from ion exchange resins and non-functional adsorbent resins.

Claims

1. A culture medium for the growth and detection in a body fluid sample of an infecting microorganism which is susceptible to antibiotics comprising:

a hypertonic growth medium capable of providing osmotic protection to the infecting microorganism which has been damaged by exposure to antibiotics and at least one resin capable of isolating the antibiotics without hindering biological activity during culturing of the body fluid sample, wherein at least one resin is not removed from the culture medium during culturing of the body fluid sample.

2. A culture medium as in claim 1 wherein the resin is an ion exchange resin.

3. A culture medium as in claim 2 wherein the resin is selected from the group consisting of cationic exchange resin and ionic exchange resin.

4. A culture medium as in claim 1 wherein the resin is a nonionic adsorbent resin.

5. A culture medium as in claim 4 wherein the resin is a nonfunctional polystyrene resin cross-linked with divinyal benzene.

6. A culture medium as in claim 1 wherein the resin is an ion exchange resin and a nonionic adsorbent resin.

7. A method for the growth and detection in a body fluid sample of an infecting microorganism which is susceptible to antibiotics, by isolating antibiotics within said body fluid sample, comprising:

a) providing a culture medium for culturing and detecting antibiotic susceptible microorganisms in a body fluid sample wherein said culture medium comprises an isotonic growth media and at least one resin capable of isolating the antibiotics without hindering biological activity during culturing of the body fluid sample, wherein said at least one resin is not removed from the culture medium during culturing of the body fluid sample;

b) inoculating said culture medium with said body fluid sample;

c) culturing said culture medium under conditions sufficient to cause production of said infecting microorganism; and

d) detecting the biological activity of the infecting microorganism.

8. The method of claim 7 wherein the resin is an ion exchange resin.

9. The method of claim 8 wherein the resin is selected from the group consisting of cationic exchange resin and ionic exchange resin.

10. The method of claim 7 wherein the resin is a nonionic adsorbent resin.

11. The method of claim 10 wherein the resin is a non-functional polystyrene resin cross-linked with divinyl benzene.

12. The method of claim 7 wherein the resin is an ion exchange resin and a nonionic adsorbent resin.