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Method for optically measuring chemical analytes

  • US 5,624,847 A
  • Filed: 08/06/1993
  • Issued: 04/29/1997
  • Est. Priority Date: 05/03/1991
  • Status: Expired due to Term
First Claim
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1. A method of optically measuring analytes, said method comprising the steps of:

  • adding a photoluminescent ligand probe to a sample to be analyzed, said sample containing an analyte which is an ionic solute, wherein said probe is non-covalently bound to said ionic solute to form an analyte-bound probe species, and wherein bound and unbound species of said probe exist in said sample, said probe having intrinsic analyte-induced lifetime changes;

    exciting the sample with radiation;

    detecting the resulting emission beam from said bound and unbound species; and

    performing a calculation consisting essentially of calculating the apparent luminescence lifetime of the emission to determine the analyte concentration of the sample.

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