Modified green fluorescent proteins
First Claim
1. A nucleic acid molecule comprising a nucleotide sequence encoding a functional mutant fluorescent protein whose amino acid sequence differs from the amino acid sequence of Aequorea green fluorescent protein (SEQ ID NO:
- 2) by at least an amino acid substitution selected from the group consisting of T203I, I167V, I167T, Y66H, Y66W, and a substitution at S65, said functional mutant having a different excitation or emission spectrum from Aequorea green fluorescent protein.
3 Assignments
0 Petitions
Accused Products
Abstract
Modifications in the sequence of Aequorea wild-type GFP provide products having markedly different excitation and emission spectra from corresponding products from wild-type GFP. In one class of modifications, the product derived from the modified GFP exhibits an alteration in the ratio of two main excitation peaks observed with the product derived from wild-type GFP. In another class, the product derived from the modified GFP fluoresces at a shorter wavelength than the corresponding product from wild-type GFP. In yet another class of modifications, the product derived from the modified GFP exhibits only a single excitation peak and enhanced emission relative to the product derived from wild-type GFP.
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Citations
25 Claims
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1. A nucleic acid molecule comprising a nucleotide sequence encoding a functional mutant fluorescent protein whose amino acid sequence differs from the amino acid sequence of Aequorea green fluorescent protein (SEQ ID NO:
- 2) by at least an amino acid substitution selected from the group consisting of T203I, I167V, I167T, Y66H, Y66W, and a substitution at S65, said functional mutant having a different excitation or emission spectrum from Aequorea green fluorescent protein.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
Specification