Transgenic non-human animals for producing heterologous antibodies
First Claim
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1. A transgenic mouse containing in its genome a transgene comprising in operable linkage a plurality of human V genes, a plurality of human D genes, a plurality of human J genes, a human μ
- CH gene, at least two different non-μ
human CH genes and associated isotype switching sequences, wherein human μ and
human γ
switch sequences are located in closer proximity to each other than in the naturally occurring human immunoglobulin locus; and
wherein in lymphocytes of said mouse the transgene undergoes productive VDJ rearrangement and μ
to γ
isotype switching by recombination between the human μ and
human γ
switch sequences such that the mouse produces a serum containing immunoglobulins of at least three human heavy chain isotypes in response to antigenic stimulation.
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Abstract
The invention relates to transgenic non-human animals capable of producing heterologous antibodies and methods for producing human sequence antibodies which bind to human antigens with substantial affinity.
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Citations
5 Claims
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1. A transgenic mouse containing in its genome a transgene comprising in operable linkage a plurality of human V genes, a plurality of human D genes, a plurality of human J genes, a human μ
- CH gene, at least two different non-μ
human CH genes and associated isotype switching sequences, wherein human μ and
human γ
switch sequences are located in closer proximity to each other than in the naturally occurring human immunoglobulin locus; and
wherein in lymphocytes of said mouse the transgene undergoes productive VDJ rearrangement and μ
to γ
isotype switching by recombination between the human μ and
human γ
switch sequences such that the mouse produces a serum containing immunoglobulins of at least three human heavy chain isotypes in response to antigenic stimulation. - View Dependent Claims (2, 3, 4, 5)
- CH gene, at least two different non-μ
Specification