Multi-test format with gel-forming matrix for characterization of microorganisms
First Claim
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1. A method for testing microorganisms selected from the group consisting of bacteria and fungi, comprising the steps of:
- a) providing a testing device comprising a plurality of testing wells, wherein said wells contain a testing substrate and one or more gel-initiating agents;
b) preparing a suspension comprising a pure culture of microorganisms and an aqueous solution comprising a gelling agent, under conditions such that said suspension remains ungelled;
c) introducing said suspension into said wells of said testing device under conditions such that said suspension forms a gel matrix within said well, such that said microorganisms are within said gel matrix; and
d) detecting the response of said microorganisms to said testing substrate.
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Abstract
The present invention is directed to methods and compositions for the characterization of various microorganisms. In particular, the present invention is suited for the characterization of commonly encountered microorganisms (e.g., E. coli, S. aureus, etc.), as well as commercially and industrially important organisms from various and diverse environments. For example, the present invention is particularly suited for the growth and characterization of the actinomycetes and fungi.
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Citations
19 Claims
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1. A method for testing microorganisms selected from the group consisting of bacteria and fungi, comprising the steps of:
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a) providing a testing device comprising a plurality of testing wells, wherein said wells contain a testing substrate and one or more gel-initiating agents; b) preparing a suspension comprising a pure culture of microorganisms and an aqueous solution comprising a gelling agent, under conditions such that said suspension remains ungelled; c) introducing said suspension into said wells of said testing device under conditions such that said suspension forms a gel matrix within said well, such that said microorganisms are within said gel matrix; and d) detecting the response of said microorganisms to said testing substrate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A kit for characterizing and identifying microorganisms selected from the group consisting of bacteria and fungi, comprising:
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i) a microplate testing device containing a plurality of wells, wherein said wells contain one or more gel-initiating agents and one or more testing substrates; and ii) an aqueous suspension comprising a gelling agent. - View Dependent Claims (10, 11, 12, 13)
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14. A method for testing microorganisms, wherein said microorganisms are selected from the group consisting of bacteria and fungi, comprising the steps of:
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a) providing a testing device comprising a plurality of testing wells, wherein said wells contain a testing substrate and one or more gel-initiating agents; b) preparing a suspension comprising a pure culture of microorganisms and an aqueous solution comprising a gelling agent, wherein said gelling agent is selected from the group consisting of gellan gum, carrageenan, and alginic acid, under conditions such that said suspension remains ungelled; c) introducing said suspension into said wells of said testing device under conditions such that said suspension forms a gel matrix within said well; and d) detecting the response of said microorganisms to said testing substrate. - View Dependent Claims (15, 16, 17, 18, 19)
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Specification