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Methods for the production of proteins with a desired function

  • US 5,627,052 A
  • Filed: 06/05/1995
  • Issued: 05/06/1997
  • Est. Priority Date: 08/02/1990
  • Status: Expired due to Term
First Claim
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1. A method for producing an antibody, antibody variable region, or a portion thereof which has an antibody binding region with a desired function, comprising:

  • (a) obtaining from a warm-blooded animal a population of antibody-forming B cells or antibody-forming progeny thereof wherein the B cells or progency thereof are non-hybridoma or non-Epstein Barr virus (EBV) transformed, and wherein the B cells or progency thereof contain at least one cell that produces an antibody exhibiting a desired function, followed by;

    (b) suspending the population of antibody-forming B cells or progency thereof in a medium, the medium having an indicator system incorporated therein which indicates the presence and location of a cell which forms antibodies exhibiting the desired function;

    (c) identifying a cell forming an antibody exhibiting the desired function;

    (d) isolating the identified antibody-forming cell from the medium;

    (e) determining the amino acid sequence of the variable region or a portion thereof which exhibits the desired function of the antibody produced by the isolated antibody-forming cell; and

    (f) synthesizing an antibody, antibody variable region, or a portion thereof which has the amino acid sequence of the variable region or portion thereof which exhibits the desired function.

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