Enzymatic hydrolysis of biomass material

• US 5,628,830 A
• Filed: 06/06/1995
• Issued: 05/13/1997
• Est. Priority Date: 03/23/1979
• Status: Expired due to Term

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Brink, David L.
• Primary Examiner(s): Caldarola, Glenn A.
• Assistant Examiner(s): HAILEY, PATRICIA L
• Application Number:
  US08/465,840
• Time in Patent Office: 707 Days
• Field of Search: 127/36, 127/37, 127/43, 162/25, 162/81
• US Class Current: 127/36
• CPC Class Codes: C12P 7/10 substrate containing cellulosic material
• CPC Class Codes: C13K 1/02 obtained by saccharification of cellulosic materials manufacture of fodder A23K10/32
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

A method of producing sugars and converting the sugars to ethanol in which particulate biomass resulting from hydrolysis of the hemicellulosic content of the biomass is subjected to enzymatic hydrolysis of the cellulosic content to glucose and fermentation of the glucose to ethanol. The particulate biomass resulting from hydrolysis of the hemicellulosic content of the feed stock is reduced to very fine particle size before enzymatic hydrolysis. The hydrolysis of cellulose and the fermentation of the resulting glucose may be carried out separately or they may be carried out simultaneously. The pentoses and hexoses resulting from hydrolysis of the hemicellulose may be subjected to fermentation separately to produce ethanol or they may be fermented simultaneously with the fermentation of the glucose derived from the cellulose.

Claims

1. A method of hydrolysis of biomass material containing hemicellulosic, cellulosic and ligneous components which comprises subjecting the biomass material to hydrolysis under conditions to hydrolyze the hemicellulose content without substantial hydrolysis of the cellulosic content, then subjecting residual solid biomass in finely divided particulate form to enzymatic hydrolysis to convert the cellulose to glucose.

2. The method of claim 1 wherein the resulting glucose is fermented to ethanol.

3. The method of claim 2 wherein the enzymatic hydrolysis and the fermentation are carried out simultaneously.

4. The method of any one of claims 1, 2 or 3 wherein the finely divided particulate residual solid biomass is subjected to decompressive explosion through an orifice to further enhance the cellulose to enzymatic hydrolysis to glucose.

5. The method of claim 4, wherein the aqueous phase and particulate solids, after decompressive explosion, are subjected to simultaneous enzymatic hydrolysis and fermentation of both hexose and pentose sugars to yield ethanol.