Vibrio cholerae strain CVD103Hg.sup.r, method of making same, and vaccines derived therefrom
First Claim
1. A vaccine for protecting against cholera comprising Vibrio cholerae having the identifying characteristics of Vibrio cholerae CVD103Hgr (ATCC No. 55,456).
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Abstract
Method of isolating deletion mutants of Vibrio cholerae, wherein the deletion is predetermined by digestion with restriction endonucleases of known specificity. The deletions are inserted into the Vibrio cholerae chromosome by in vivo recombination between a plasmid carrying the desired deletion, with adjacent flanking sequences, and the Vibrio cholerae chromosome. The invention includes the isolation and characterization of a new Vibrio cholerae strain, CVD103Hg (ATTC No. 55,456), having a deletion in the tox gene, as defined by Acc I, Xba I, Cla I and/or restriction endonuclease sites, and carrying a mercury resistance gene. The invention also includes vaccines for protecting against the symptoms of cholera as well as methods for achieving this protection.
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Citations
28 Claims
- 1. A vaccine for protecting against cholera comprising Vibrio cholerae having the identifying characteristics of Vibrio cholerae CVD103Hgr (ATCC No. 55,456).
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15. A pharmaceutical composition comprising:
- Vibrio cholerae having the identifying characteristics of Vibrio cholerae CVD103Hgr (ATCC No. 55,456) and a pharmaceutically acceptable carrier.
- View Dependent Claims (16, 17, 18, 19, 20)
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21. A method of protecting humans against cholera comprising:
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obtaining a Vibrio cholerae culture comprising a Vibrio cholerae having substantially all of the identifying characteristics of Vibrio cholerae CVD103Hgr (ATCC No. 55,456); and administering an effective amount of said culture to a human. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28)
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Specification