×

Solid phase diagnosis of medical conditions

  • US 5,629,158 A
  • Filed: 06/07/1995
  • Issued: 05/13/1997
  • Est. Priority Date: 03/22/1989
  • Status: Expired due to Fees
First Claim
Patent Images

1. A method for detecting a target polynucleotide that is characteristic of a pathological condition, comprising the steps of:

  • (a) producing a first amplified DNA by a first polymerass chain reaction using a first set of primers to amplify a target polynucleotide characteristic of a pathological condition;

    (b) producing from said first amplified DNA a second amplified DNA by a second polymerass chain reaction using a second set of primers, wherein;

    (i) said second primers differ from said first primers,(ii) a 5'"'"' region of said second primers is not complementary to said first amplified DNA,(iii) one of said second primers is biotinylated, and(iv) another of said second primers comprises means for binding a detectable label;

    (c) binding said biotinylated second primer to a solid support before, during or after said second polymerase chain reaction, whereby said second amplified DNA is bound to said support;

    (d) binding a detectable label to said means for binding a detectable label after said second polymerase chain reaction, whereby said detectable label is bound to said second amplified DNA, wherein said second amplified DNA is double-stranded; and

    (e) detecting said target polynucleotide by determining the detectable label bound to said double-stranded second amplified DNA.

View all claims
  • 0 Assignments
Timeline View
Assignment View
    ×
    ×