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Enzyme immunoassay device

  • US 5,629,164 A
  • Filed: 11/25/1992
  • Issued: 05/13/1997
  • Est. Priority Date: 11/14/1990
  • Status: Expired due to Fees
First Claim
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1. An enzyme immunoassay device for use in determining the presence of an analyte in a sample which comprises:

  • i) a planar support comprising an upper surface and a lower surface and further comprising a fold means defining a first and a second support half by which the upper surface of the device may be folded together along said means;

    ii) a first opening in the first support half (a) spaced a defined distance from the fold means, (b) extending through the support, and (c) defining a sample well comprising an interior being opened at the lower surface of the support and closed at the upper surface of the support with a closure comprising a conjugate pad of negligible liquid absorptive qualities facing the interior of the sample well, said conjugate pad containing a mobilizable immunoreactant comprising said analyte conjugated to an enzyme; and

    an antibody pad having greater liquid absorptivity than said conjugate pad, comprising an immobilized antibody that specifically binds to said analyte, and located axial to the conjugate pad and at the upper surface of the support; and

    iii) a second opening located at the upper surface of the second support half (a) spaced an equal distance to the first opening from the fold means, (b) extending partially through the support, and (c) containing a readout pad comprising a chromophoric indicator means that reacts with said enzyme to produce a color change, and wherein the antibody and readout pads will be in contact with each other when the support is folded along the fold means.

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