Methods for forming an optical device for detecting the presence or amount of an analyte
First Claim
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1. A method for forming an optical device for detecting the presence or amount of an analyte of interest comprising:
- a substrate which supports an optically active layer, comprising an optical thin film,an attachment layer provided on said optically active layer, wherein said attachment layer comprises a material selected from the group consisting of dendrimers, star polymers, molecular self-assembling polymers, polymeric siloxanes, and film forming latexes, anda receptive layer specific for said analyte provided on said attachment layer, said method comprising the steps of;
forming said optical thin film with a chosen refractive index on said substrate by curing the optical thin film on the substrate at a controlled temperature or for a controlled length of time such that said optically active layer in conjunction with said attachment and receptive layers exhibits a first color in response to light impinging thereon, and exhibits a second color comprising a combination of wavelengths of light different from said first color or comprising an intensity of at least one said wavelength different from said first color, in response to said light when said analyte is present on said receptive layer; and
subsequently providing said attachment and receptive layers on said optically active layer.
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Abstract
A method for forming an optical device for detecting the presence or amount of an analyte of interest comprising a substrate which supports an optically active layer, an attachment layer provided on the optically active layer, and a receptive layer specific the analyte provided on the attachment layer. The method comprises forming the optically active layer with a chosen refractive index on the substrate by curing the optically active layer on the substrate at a controlled temperature or for a controlled length of time and subsequently providing the attachment and receptive layers on the optically active layer.
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Citations
30 Claims
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1. A method for forming an optical device for detecting the presence or amount of an analyte of interest comprising:
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a substrate which supports an optically active layer, comprising an optical thin film, an attachment layer provided on said optically active layer, wherein said attachment layer comprises a material selected from the group consisting of dendrimers, star polymers, molecular self-assembling polymers, polymeric siloxanes, and film forming latexes, and a receptive layer specific for said analyte provided on said attachment layer, said method comprising the steps of; forming said optical thin film with a chosen refractive index on said substrate by curing the optical thin film on the substrate at a controlled temperature or for a controlled length of time such that said optically active layer in conjunction with said attachment and receptive layers exhibits a first color in response to light impinging thereon, and exhibits a second color comprising a combination of wavelengths of light different from said first color or comprising an intensity of at least one said wavelength different from said first color, in response to said light when said analyte is present on said receptive layer; and
subsequently providing said attachment and receptive layers on said optically active layer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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22. A method for forming a device for use in an optical assay for an analyte comprising:
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a multi-layered substrate comprising a layer of base material, a conducting metal layer on said layer of base material comprising aluminum, chromium, or a transparent conducting oxide, a layer of amorphous silicon on said conducting metal layer, an anti-reflective layer on said layer of amorphous silicon, an attachment layer on said anti-reflective layer, wherein said attachment layer comprises a material selected from the group consisting of dendrimers, star polymers, molecular self-assembling polymers, polymeric siloxanes, and film forming latexes, and a receptive layer specific for said analyte on said attachment layer, said method comprising the steps of; providing said layers of conducting metal and amorphous silicon on said layer of base material, forming said anti-reflective layer with a chosen refractive index on said layer of amorphous silicon by curing the anti-reflective layer at a controlled temperature or for a controlled length of time, and subsequently providing said attachment and receptive layers on said anti-reflective layer. - View Dependent Claims (24, 25, 27, 28, 29, 30)
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23. A method for forming a device for use in an optical assay for an analyte comprising:
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a multi-layered substrate comprising a layer of base material, a layer of amorphous silicon on said layer of base material, and an an anti-reflective layer on said layer of amorphous silicon, an attachment layer on said anti-reflective layer, wherein said attachment layer comprises a material selected from the group consisting of dendrimers, star polymers, molecular self-assembling polymers, polymeric siloxanes, and film forming latexes, and a receptive layer specific for said analyte on said attachment layer, said method comprising the step of; forming said anti-reflective layer with a chosen refractive index on said layer of amorphous silicon by curing the anti-reflective layer at a controlled temperature or for a controlled length of time, and subsequently providing said attachment and receptive layers on said anti-reflective layer.
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26. A method for forming an optical assay device for an analyte comprising:
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a substrate selected from the group consisting of glass, plastic, silicon, and amorphous silicon, an anti-reflective layer on said substrate selected from the group consisting of silicon nitride, composite of silicon/silicon dioxide, titanates, silicon carbide, diamond, cadmium sulfide, and titanium dioxide, an attachment layer on said anti-reflective layer selected from the group consisting of a polymeric silane, siloxane, film forming latex, and a dendrimer, and a specific binding layer for said analyte on said attachment layer, said anti-reflective layer comprising an optical thin film, said method comprising the step of; forming said optical thin film on said substrate with a chosen refractive index by curing said optical thin film on the substrate at a controlled temperature or for a controlled length of time, and subsequently providing said attachment and receptive layers on said optical thin film.
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Specification