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Methods of preparing probe array by hybridation

  • US 5,631,134 A
  • Filed: 06/05/1995
  • Issued: 05/20/1997
  • Est. Priority Date: 11/06/1992
  • Status: Expired due to Term
First Claim
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1. A method for creating a nucleic acid probe array comprising the steps of:

  • a) hybridizing a plurality of single-stranded first nucleic acids to a plurality of longer, single-stranded second nucleic acids complementary to the first nucleic acids wherein each second nucleic acid contains a variable terminal nucleotide sequence to form probes each having a double-stranded portion and a single-stranded portion with the variable nucleotide sequence in the single-stranded portion;

    b) hybridizing target nucleic acids to the probes;

    c) ligating hybridized targets to the first nucleic acids of the probes;

    d) isolating the second nucleic acids; and

    e) hybridizing additional first nucleic acids to isolated second nucleic acids to form the nucleic acid probe array.

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