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Oligonucleotide N3'.fwdarw.P5' phosphoramidates: hybridization and nuclease resistance properties

  • US 5,631,135 A
  • Filed: 06/06/1995
  • Issued: 05/20/1997
  • Est. Priority Date: 03/18/1994
  • Status: Expired due to Term
First Claim
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1. A method of hybridizing an oligodeoxyribonucleotide to an RNA target, comprising:

  • (a) forming an oligodeoxyribonucleotide comprising a sequence of nucleoside subunits joined by inter-subunit linkages, wherein;

    (i) at least 50% of the inter-subunit linkages of said oligodeoxyribonucleotide are N3'"'"'→

    P5'"'"' phosphoramidate inter-subunit linkages of the formula;

    
    
    space="preserve" listing-type="equation">3'"'"'-NH-P(═

    O)(-O.sup.-)-O-5'"'"'; and

    (ii) said oligodeoxyribonucleotide has at least 11 nucleotides; and

    (b) contacting the oligodeoxyribonucleotide with said RNA target to allow formation of a hybridization complex between said oligodeoxyribonucleotide and said RNA target.

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