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Method for the quantitative determination of glycated proteins

  • US 5,631,140 A
  • Filed: 07/17/1995
  • Issued: 05/20/1997
  • Est. Priority Date: 07/18/1994
  • Status: Expired due to Term
First Claim
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1. A method for the determination of a glycated protein relative to non-glycated protein in an aqueous sample, comprising:

  • reacting the sample with a proteolytic enzyme to form a peptide mixture comprising an amount of glycated peptides and an amount of non-glycated peptides,separating said glycated peptides and said non-glycated peptides in said peptide mixture, anddetermining said glycated protein relative to said non-glycated protein by measuring the amount of said glycated peptides and the amount of said non-glycated peptides and comparing the amount of said glycated peptides with the amount of said non-glycated peptides.

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