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Fluorescent energy transfer immunoassay

  • US 5,631,169 A
  • Filed: 01/19/1994
  • Issued: 05/20/1997
  • Est. Priority Date: 01/17/1992
  • Status: Expired due to Term
First Claim
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1. A competitive method of quantifying an analyte in a sample, consisting essentially of the steps of:

  • adding to said sample a first binding partner and a second binding partner, wherein said first binding partner competes with the analyte for binding to said second binding partner, wherein one of said first and second binding partners is labelled with a photoluminescent energy transfer donor and the other is labelled with a photoluminescent energy transfer acceptor, wherein the photoluminescent energy transfer donor and acceptor are chosen such that when the first binding partner binds to the second binding partner, the donor and the acceptor are brought into interacting proximity, producing a detectable luminescence lifetime change in the photoluminescence lifetime of the donor;

    exposing the sample to an exciting amount of radiation;

    detecting the resulting emission; and

    calculating the apparant luminescence lifetime of the donor to quantify binding of the first binding partner to the second binding partner, thereby inversely quantifying the analyte.

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