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Method for improving measurement precision in evanescent wave optical biosensor assays

  • US 5,631,170 A
  • Filed: 12/12/1994
  • Issued: 05/20/1997
  • Est. Priority Date: 06/10/1992
  • Status: Expired due to Term
First Claim
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1. A method of improving measurement precision in an optical biosensor assay for a ligand in a sample which comprises the steps ofi) incubating the sample in contact with a measurement surface which carries a directly or indirectly immobilised measurement reagent appropriate to the assay and which additionally carries an amount of a directly or indirectly immobilised reference reagent which gives rise to a reference signal, independent of the amount of ligand present in the sample, at the measurement surface, and prior to, during, or subsequent to the said incubation of the sample measuring said reference signal by a method appropriate to the assay;

  • ii) simultaneously with or sequentially to the said incubation of the sample in i) introducing one or more labelled ancillary reagents appropriate to the assay whereby if ligand is present in the sample, a complex involving said measurement reagent and at least one of said ligand or said labelled ancillary reagent(s) is formed giving rise to a detectable assay signal which is a first function of the amount of ligand (if any) present in the sample; and

    iii) subsequently monitoring the assay signal arising from the measurement surface by a method appropriate to the assay and, comparing the reference signal with the assay signal, thereby determining using an appropriate algorithm whether and/or the extent to which the ligand under assay is present in the sample.

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