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Pseudo telecentric optical design for flow cytometric blood cell analyzer

  • US 5,631,730 A
  • Filed: 07/28/1995
  • Issued: 05/20/1997
  • Est. Priority Date: 08/01/1994
  • Status: Expired due to Term
First Claim
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1. A flow cytometric optical system for the simultaneous detection of several characteristics of particles suspended in a flowing medium comprising:

  • a flowcell through which the particles pass substantially one particle at a time;

    a light directing optical system for directing light from a light source onto the flowing particles in the flowcell;

    a side angle optical collection system for receiving light from the flowing particles, and for directing the light to one or more of a first set of detectors;

    a forward angle collection system for receiving light from the flowing particles, and for directingthe light to one or more of a second set of detectors;

    wherein the side angle optical collection system comprises a condenser lens for directing light toward the first set of detectors, an exit pupil of the condenser lens located at the back focal plane of the condenser lens, and a photosensitive surface of one or more of the first set of detectors located at conjugate points of the back focal plane of the condenser lens such that an image of the exit pupil is positioned at the photosensitive surface of the one or more of the first set of detectors; and

    wherein the forward angle optical collection system comprises a collecting lens for directing light toward the second set of detectors, wherein the collecting lens exit pupil located in the back focal plane of the collecting lens, a photosensitive surface of one or more of the second set of detectors located at the back focal plane of the collecting lens.

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