Pseudo telecentric optical design for flow cytometric blood cell analyzer

• US 5,631,730 A
• Filed: 07/28/1995
• Issued: 05/20/1997
• Est. Priority Date: 08/01/1994
• Status: Expired due to Term

• Current Assignee: Abbott Laboratories
• Original Assignee: Abbott Laboratories
• Inventors: Mehta, Suresh N., Chupp, Vernon L.
• Primary Examiner(s): Rosenberger, Richard A.
• Application Number:
  US08/508,502
• Time in Patent Office: 662 Days
• Field of Search: 356/72, 356/73, 356/339, 356/338
• US Class Current: 356/73
• CPC Class Codes: B01F 25/3132 by using two or more injector devices
• CPC Class Codes: B01F 25/31324 arranged concentrically
• CPC Class Codes: G01N 1/14 Suction devices, e.g. pumps; Ejector devices
• CPC Class Codes: G01N 15/14 Optical investigation techniques, e.g. flow cytometry
• CPC Class Codes: G01N 15/1404 Handling flow, e.g. hydrodynamic focusing
• CPC Class Codes: G01N 2015/014 Reticulocytes
• CPC Class Codes: G01N 2015/018 Platelets
• CPC Class Codes: G01N 2015/1413 Hydrodynamic focussing
• CPC Class Codes: G01N 2035/00356 Holding samples at elevated temperature (incubation)
• CPC Class Codes: G01N 2035/00524 Mixing by agitating sample carrier
• CPC Class Codes: G01N 2035/1032 Dilution or aliquotting
• CPC Class Codes: G01N 35/0092 Scheduling
• CPC Class Codes: G01N 35/10 Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices G01N35/0099 takes precedence
• CPC Class Codes: G01N 35/1004 Cleaning sample transfer devices
• CPC Class Codes: G01N 35/109 with two horizontal degrees of freedom

Abstract

A pseudo telecentric flow cytometric optical system for the simultaneous detection of several characteristics of particles suspended in a flowing medium. The system is made up of a flowcell through which the particles pass substantially one particle at a time, an optical system for directing light from a light source onto the flowing particles in the flowcell, a side angle optical collection system for receiving light from the flowing particles and for directing the light to one or more of a first set of detectors, and a forward angle collection system for receiving light from the flowing particles and for directing the light to one or more of a second set of detectors. The side angle optical collection system can include a condenser lens for directing light toward the first set of detectors with an exit pupil of the condenser lens located at the back focal plane of the condenser lens; a photosensitive surface of one or more of the first set of detectors is located at conjugate points of the back focal plane of the condenser lens such that an image of the exit pupil is positioned at the photosensitive surface of one or more of the first set of detectors. The forward angle optical collection system can include a collecting lens for directing light toward the second set of detectors where the collecting lens exit pupil is located in the back focal plane of the collecting lens and a photosensitive surface of one or more of the second set of detectors is located at the back focal plane of the collecting lens.

Claims

1. A flow cytometric optical system for the simultaneous detection of several characteristics of particles suspended in a flowing medium comprising:

a flowcell through which the particles pass substantially one particle at a time;

a light directing optical system for directing light from a light source onto the flowing particles in the flowcell;

a side angle optical collection system for receiving light from the flowing particles, and for directing the light to one or more of a first set of detectors;

a forward angle collection system for receiving light from the flowing particles, and for directingthe light to one or more of a second set of detectors;

wherein the side angle optical collection system comprises a condenser lens for directing light toward the first set of detectors, an exit pupil of the condenser lens located at the back focal plane of the condenser lens, and a photosensitive surface of one or more of the first set of detectors located at conjugate points of the back focal plane of the condenser lens such that an image of the exit pupil is positioned at the photosensitive surface of the one or more of the first set of detectors; and

wherein the forward angle optical collection system comprises a collecting lens for directing light toward the second set of detectors, wherein the collecting lens exit pupil located in the back focal plane of the collecting lens, a photosensitive surface of one or more of the second set of detectors located at the back focal plane of the collecting lens.

2. The optical system of claim 1 wherein the light source is a laser.

3. The optical system of claim 2 wherein the second set of detectors comprises an array detector comprising two or more elements, each element defining an angular acceptance range of light scattered by the particles as determined by the radial linear limiting dimensions of that element.

4. The optical system of claim 3 wherein one element of the array detector has physical sensitivity dimensions corresponding to the laser beam divergence within the flowcell in the absence of any particles.

5. The optical system of claim 2 wherein the laser has a focal waist focused within the flowcell.

6. The optical system of claim 2 wherein the light directing optical system comprises a fine adjust element to enable a lateral translation or the laser beam within the flowcell without adversely effecting the trajectory of the incident laser beam.

7. The optical system of claim 6 wherein the fine adjust element comprises a pair of wedge prisms, separated by an adjustable air space, the pair of prisms located between a laser focusing lens and the flowcell.

8. The optical system of claim 1 wherein the first set of detectors comprises one or more photomultipler tubes.

9. The optical system of claim 1 wherein the condenser lens is a compound lens.

10. The optical system of claim 9 wherein the exit pupil of the compound condenser lens is virtual and located within the lens.

11. The optical system of claim 1 wherein the second set of detectors comprises one or more photodiodes.

12. The optical system of claim 1 wherein the second set of detectors comprises a single silicon photodiode with an angular acceptance range of the light scattered from the particles is determined by the linear dimensions of the photosensitive surface of the photodiode.

13. The optical system of claim 1 wherein the condenser lens forms at least one image of the flowcell medium/particle stream at a position located between the condenser and the first set of detectors.

14. The optical system of claim 13 wherein the side angle optical system further comprises at least one aperture located at the at least one flowcell medium/particle stream image, the aperture limiting light to the one or more of the first set of detectors.

15. The optical system of claim 14 wherein the dimensions of the aperture are greater than the dimensions of the flowcell medium/particle stream such that the defocusing effects of stream wander within the flowcell, flowcell replacement or the replacement of the medium/particle stream forming means does not adversely affect the signal received at a detector.

16. The optical system of claim 14 wherein the side angle optical collection system further comprises a field lens located at or near the aperture, the field lens producing an image of the condenser exit pupil at the photosensitive surface of at least one of the first set of detectors.

17. The optical flowcell of claim 1 wherein the field lens comprises a compound lens.

18. A flow cytometric optical system for the simultaneous detection of several characteristics of particles suspended in a flowing medium comprising:

a flowcell through which the particles pass;

an optical system for directing light from a light source onto the flowing particles in the flowcell;

a side angle optical collection system for receiving light from the flowing particles, and for directing the light to one or more of a first set of detectors;

a forward angle collection system for receiving light from the flowing particles, and for directing said light to one or more of a second set of detectors;

wherein the side angle optical collection system comprises a condenser lens for directing light toward the first set of detectors, an exit pupil of said condenser lens located at the back focal plane of said condenser lens, and a photosensitive surface of one or more of said first set of detectors being located at conjugate points of the back focal plane of said condenser lens, such that an image of said exit pupil is positioned at the photosensitive surface of one or more of the first set of detectors; and

wherein the forward angle optical collection system comprises a collecting lens for directing light toward the second set of detectors, an exit pupil of the collecting lens located in the back focal plane of the collecting lens, and a photosensitive surface of one or more of the second set of detectors being located at conjugate points of the back focal plane of said collecting lens, such that an image of said exit pupil is located at the photosensitive surface of one or more of the second set of detectors.

19. The optical system of claim 18 wherein the light source is a laser.

20. The optical system of claim 19 wherein the second set of detectors comprises an array detector comprising two or more elements, each element defining an angular acceptance range of light scattered by the particles as determined by the radial linear limiting dimensions of that element.

21. The optical system of claim 20 wherein one element of the array detector has physical sensitivity dimensions corresponding to the laser beam divergence within the flowcell in the absence of any particles.

22. The optical system of claim 19 wherein the laser has a focal waist focused within the flowcell.

23. The optical system of claim 19 wherein the light directing optical system comprises a fine adjust element to enable a lateral translation or the laser beam within the flowcell without adversely effecting the trajectory of the incident laser beam.

24. The optical system of claim 23 wherein the fine adjust element comprises a pair of wedge prisms, separated by an adjustable air space, the pair of prisms located between a laser focusing lens and the flowcell.

25. The optical system of claim 18 wherein the first set of detectors comprises one or more photomultipler tubes.

26. The optical system of claim 18 wherein the condenser lens is a compound lens.

27. The optical system of claim 26 wherein the exit pupil of the compound condenser lens is virtual and located within the lens.

28. The optical system of claim 18 wherein the second set of detectors comprises one or more photodiodes.

29. The optical system of claim 18 wherein the second set of detectors comprises a single silicon photodiode with an angular acceptance range of the light scattered from the particles as determined by the linear dimensions of the photosensitive surface of the photodiode.

30. The optical system of claim 18 wherein the condenser lens forms at least one image of the flowcell medium/particle stream at a position located between the condenser and the first set of detectors.

31. The optical system of claim 30 wherein the side angle optical system further comprises at least one aperture located at the at least one flowcell medium/particle stream image, the aperture limiting light to the one or more of the first set of detectors.

32. The optical system of claim 31 wherein the dimensions of the aperture are greater than the dimensions of the flowcell medium/particle stream such that the defocusing effects of stream wander within the flowcell, flowcell replacement or the replacement of the medium/particle stream forming means does not adversely affect the signal received at a detector.

33. The optical system of claim 31 wherein the side angle optical collection system further comprises a field lens located at or near the aperture, the field lens producing an image of the condenser exit pupil at the photosensitive surface of at least one of the first set of detectors.

34. The optical flowcell of claim 18 wherein the field lens comprises a compound lens.