Treated tissue for implantation and methods of preparation
First Claim
1. A process for generating a substantially non-immunogenic tissue matrix suitable for subsequent processing into an implant tissue comprising:
- A. eliminating native cells by treating a tissue with components selected from the group consisting enzymes and nucleases effective to inhibit subsequent native cell growth in the treated tissue and effective to limit generation of new immunological sites in the tissue thus producing a tissue matrix;
B. treating the tissue matrix with cellular adhesion factor to promote subsequent attachment of cultured allogeneic or autologous cells to the surfaces of the tissue matrix; and
C. repopulating the tissue matrix throughout the matrix with cultured allogeneic or autologous cells.
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Abstract
This disclosure includes a method for generating a functional hybrid bioprosthesis. Tissue formed naturally of interstitial collagens is treated to kill native cells and remove potentially immunologically active soluble molecules. Then it may be treated sequentially with extracellular matrix adhesion factor, extracellular matrix glycosaminoglycan, and growth factor appropriate to the cell type required to function within the matrix, and incubating the transplant tissue matrix with cells that are either allogeneic or autologous for the recipient thereby imparting to the matrix the characteristics of the cell type and tissue selected. Tissues with a variety of functional bioactivities can thus be formed in vitro prior to graft transplantation or implantation which will exhibit reduced or no stimulation of an immunological response in the recipient.
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Citations
15 Claims
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1. A process for generating a substantially non-immunogenic tissue matrix suitable for subsequent processing into an implant tissue comprising:
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A. eliminating native cells by treating a tissue with components selected from the group consisting enzymes and nucleases effective to inhibit subsequent native cell growth in the treated tissue and effective to limit generation of new immunological sites in the tissue thus producing a tissue matrix; B. treating the tissue matrix with cellular adhesion factor to promote subsequent attachment of cultured allogeneic or autologous cells to the surfaces of the tissue matrix; and C. repopulating the tissue matrix throughout the matrix with cultured allogeneic or autologous cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification